MicroRNA-215 as a Diagnostic Marker in Egyptian Patients with Hepatocellular Carcinoma

Background: MicroRNAs are mentioned as a small non-coding RNAs groups and aberrant miRNA expression was found in hepatocellular carcinoma (HCC) patients. Aim: To evaluate role of plasma MicroRNA-215 as a diagnostic tool in HCC patients. Methods: A prospective study included 195 subjects: healthy con...

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Published in:	Asian Pacific journal of cancer prevention : APJCP Vol. 20; no. 9; pp. 2723 - 2731
Main Authors:	El Mahdy, Hussein Ahmed, Abdelhamid, Ismail Abdelshafy, Amen, Ahmed Ibrahim, Abdelsameea, Eman, Hassouna, Mona M
Format:	Journal Article
Language:	English
Published:	Thailand West Asia Organization for Cancer Prevention 01-09-2019
Subjects:	Adult Aged alpha-Fetoproteins - analysis Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Carcinoma, Hepatocellular - blood Carcinoma, Hepatocellular - diagnosis Carcinoma, Hepatocellular - epidemiology Carcinoma, Hepatocellular - genetics Case-Control Studies Egypt - epidemiology Female Follow-Up Studies Humans Liver Neoplasms - blood Liver Neoplasms - diagnosis Liver Neoplasms - epidemiology Liver Neoplasms - genetics Male MicroRNAs - blood MicroRNAs - genetics Middle Aged Prognosis Prospective Studies ROC Curve Egypt chronic hepatitis C virus (chronic HCV) hepatocellular carcinoma (HCC) MicroRNA-215 (miR-215)
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Summary:	Background: MicroRNAs are mentioned as a small non-coding RNAs groups and aberrant miRNA expression was found in hepatocellular carcinoma (HCC) patients. Aim: To evaluate role of plasma MicroRNA-215 as a diagnostic tool in HCC patients. Methods: A prospective study included 195 subjects: healthy controls (group I), cirrhotic patients (group II), and patients with HCC (group III). Clinical examination, radiological and laboratory investigations which included quantification of miR-215 by Real-time qPCR were done for all cases. Results: Spearman’s rank correlation revealed that in HCC group, there was a negative correlation between MiRNA-215 and serum AFP levels and focal size lesion (cm) (rs = -0.72, - 0.94 respectively, p<0.001). Receiver operating characteristics analysis for discrimination between cirrhosis and HCC groups regarding microRNA-215 displayed 78.3% sensitivity, 88.0% specificity at cutoff value of ≤ 1.90. Area under the curve (AUC) was 0.87 (p< 0.001). As regards AFP, it had a sensitivity of 81.7%, a specificity of 66.7 at cutoff value of ≥ 11.50 (ng/mL). Conclusions: Plasma level of miR-215 may be a promising biomarker in HCC diagnosis. Moreover, if miR-215 combined with AFP, it can be used as a diagnostic biomarker, for early detection of HCC.
ISSN:	1513-7368 2476-762X
DOI:	10.31557/APJCP.2019.20.9.2723