The Antibacterial Potential of Brazilian Red Propolis against the Formation and Eradication of Biofilm of Helicobacter pylori

is associated with gastrointestinal diseases, and its treatment is challenging due to antibiotic-resistant strains, necessitating alternative therapies. Brazilian red propolis (BRP), known for its diverse bioactive compounds with pharmaceutical properties, was investigated for its anti- activity, fo...

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Bibliographic Details
Published in:	Antibiotics (Basel) Vol. 13; no. 8; p. 719
Main Authors:	Santiago, Mariana B, Tanimoto, Matheus H, Ambrosio, Maria Anita L V, Veneziani, Rodrigo Cassio S, Bastos, Jairo K, Sabino-Silva, Robinson, Martins, Carlos Henrique G
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 01-08-2024 MDPI
Subjects:	Antibacterial agents antibiofilm Antibiotic resistance Antibiotics Assaying Bacteria Bioactive compounds Biofilms Brazilian red propolis Cell viability Control Drug resistance Eradication Flavonoids Gastrointestinal diseases Health aspects Helicobacter pylori Leakage Microbial mats Minimum inhibitory concentration Natural products nucleotide leakage Nucleotides Pharmacology, Experimental Physiological aspects Propolis time–kill Virulence Brazil time–kill natural products Brazilian red propolis Helicobacter pylori nucleotide leakage antibiofilm
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Summary:	is associated with gastrointestinal diseases, and its treatment is challenging due to antibiotic-resistant strains, necessitating alternative therapies. Brazilian red propolis (BRP), known for its diverse bioactive compounds with pharmaceutical properties, was investigated for its anti- activity, focusing on biofilm formation inhibition and eradication. BRP was tested against (ATCC 43526) using several assays: time-kill, nucleotide leakage, biofilm formation inhibition (determining the minimum inhibitory concentration of biofilm of 50%-MICB , and cell viability), and biofilm eradication (determining the minimum eradication concentration of biofilm of 99.9%-MBEC). Standardization of biofilm formation was also conducted. In the time-kill assay, BRP at 50 µg/mL eliminated all cells after 24 h. The nucleotide leakage assay showed no significant differences between control groups and BRP-treated groups at 25 µg/mL and 50 µg/mL. formed biofilms in vitro at 10 CFU/mL after 72 h. The MICB of BRP was 15.6 µg/mL, and at 500, 1000, and 2000 µg/mL, BRP eradicated all bacterial cells. The MBEC was 2000 µg/mL. These findings suggest that BRP has promising anti- activity, effectively inhibiting and eradicating biofilms. Further studies are necessary to elucidate BRP's mechanisms of action against .
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2079-6382 2079-6382
DOI:	10.3390/antibiotics13080719