Lysine-based polycation:heparin coacervate for controlled protein delivery

Lysine-based polycation:heparin coacervate for controlled protein delivery

Polycations have good potential as carriers of proteins and genetic material. However, poor control over the release rate and safety issues currently limit their use as delivery vehicles. Here we introduce a new lysine-based polycation, poly(ethylene lysinylaspartate diglyceride) (PELD), which exhib...

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Bibliographic Details
Published in:Acta biomaterialia Vol. 10; no. 1; pp. 40 - 46
Main Authors: Johnson, Noah Ray, Ambe, Trisha, Wang, Yadong
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-01-2014
Subjects:
Alterations
Animals
Biomedical materials
Cattle
Cell Death - drug effects
Coacervate
Controlled release
Diglycerides
Efficiency
Fibroblasts - cytology
Fibroblasts - drug effects
Genetics
Heparin
Heparin - chemical synthesis
Heparin - chemistry
Hydrodynamics
Lysine
Lysine - chemical synthesis
Lysine - chemistry
Mice
Microscopy, Electron, Scanning
Microscopy, Fluorescence
NIH 3T3 Cells
Polyamines - chemistry
Polycation
Polymers - chemistry
Proteins
Serum Albumin, Bovine - metabolism
Static Electricity
Surface charge
Tissue engineering
Tissue Scaffolds - chemistry
Coacervate
Polycation
Heparin
Lysine
Controlled release
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Summary:Polycations have good potential as carriers of proteins and genetic material. However, poor control over the release rate and safety issues currently limit their use as delivery vehicles. Here we introduce a new lysine-based polycation, poly(ethylene lysinylaspartate diglyceride) (PELD), which exhibits high cytocompatibility. PELD self-assembles with the biological polyanion heparin into a coacervate that incorporates proteins with high loading efficiency. Coacervates of varying surface charge were obtained by simple alteration of the PELD:heparin ratio and resulted in diverse release profiles of the model protein bovine serum albumin. Therefore, coacervate charge represents a direct means of control over release rate and duration. The PELD coacervate also rapidly adsorbed onto a porous polymeric scaffold, demonstrating potential use in tissue engineering applications. This coacervate represents a safe and tunable protein delivery system for biomedical applications.
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ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2013.09.012