Isolation and Characterization of Cyniclomyces guttulatus (Robin) Van Der Walt and Scott, 1971 in Dogs in Brazil

Isolation and Characterization of Cyniclomyces guttulatus (Robin) Van Der Walt and Scott, 1971 in Dogs in Brazil

Vegetative cells of an ascomycetous yeast, morphologically consistent with published descriptions of Cyniclomyces guttulatus, were observed in large numbers in the feces and stomach washes of three dogs with a recurrent medical history characterized by vomiting and diarrhea. Nucleotide sequence anal...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology Vol. 65; no. 5; pp. 542 - 546
Main Authors: Flausino, Gilberto, Leal, Paulo D. S, McIntosh, Douglas, Amaral, Luciana G, Teixeira Filho, Walter L, Flausino, Walter, Lopes, Carlos W. G
Format: Journal Article
Language:English
Published: New York Springer-Verlag 01-11-2012
Springer Nature B.V
Subjects:
Animal diseases
Animals
Biomedical and Life Sciences
Biotechnology
Brazil
diarrhea
Diarrhea - microbiology
Diarrhea - veterinary
Dog Diseases - microbiology
Dogs
Feces
Feces - microbiology
Gastrointestinal diseases
Gastrointestinal tract
Life Sciences
Microbiology
pathogens
Pathology
ribosomal DNA
sequence analysis
stomach
Stomach - microbiology
vegetative cells
vomiting
Yeast
Yeasts
Yeasts - classification
Yeasts - genetics
Yeasts - isolation & purification
Brazil
Labrador Retriever
Bromothymol Blue
Ascomycetous Yeast
Golden Retriever
Gastrointestinal Illness
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vegetative cells of an ascomycetous yeast, morphologically consistent with published descriptions of Cyniclomyces guttulatus, were observed in large numbers in the feces and stomach washes of three dogs with a recurrent medical history characterized by vomiting and diarrhea. Nucleotide sequence analysis of an approximately 600 base pair fragment of the variable D1/D2 domain of large subunit (26S) ribosomal DNA of a pure culture, isolated from a Siberian Husky, revealed 98–99 % homology to sequences deposited in the GenBank as C. guttulatus. These data represent the first observation of C. guttulatus in association with canine gastrointestinal illness in the southern hemisphere and add weight to the hypothesis that this yeast may act as an opportunistic pathogen of dogs. An extended examination of wet mounts and smears prepared from feces collected from 63 dogs with no clinical symptoms of gastrointestinal illness, identified C. gluttulatus in 14 (22.2 %) of the animals, albeit at lower numbers than in diseased dogs, indicating that this yeast species is widely distributed as a component of the normal microflora of the canine gastrointestinal tract.
Bibliography:http://dx.doi.org/10.1007/s00284-012-0184-6
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-012-0184-6