Search Results - "Amann, Kurt J."

The Structural Basis of Actin Filament Branching by the Arp2/3 Complex by Rouiller, Isabelle, Xu, Xiao-Ping, Amann, Kurt J., Egile, Coumaran, Nickell, Stephan, Nicastro, Daniela, Li, Rong, Pollard, Thomas D., Volkmann, Niels, Hanein, Dorit

“…The actin-related protein 2/3 (Arp2/3) complex mediates the formation of branched actin filaments at the leading edge of motile cells and in the comet tails…”
Get full text

Direct Real-Time Observation of Actin Filament Branching Mediated by Arp2/3 Complex Using Total Internal Reflection Fluorescence Microscopy by Amann, Kurt J., Pollard, Thomas D.

“…Existing methods for studying actin filament dynamics have allowed analysis only of bulk samples or individual filaments after treatment with the drug…”
Get full text

Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins by Pollard, Thomas D, Blanchoin, Laurent, Amann, Kurt J, Higgs, Henry N, Marchand, Jean-Baptiste, Kaiser, Donald A

“…Most nucleated cells crawl about by extending a pseudopod that is driven by the polymerization of actin filaments in the cytoplasm behind the leading edge of…”
Get full text

The Arp2/3 complex nucleates actin filament branches from the sides of pre-existing filaments by Pollard, Thomas D, Amann, Kurt J

“…Regulated assembly of actin-filament networks provides the mechanical force that pushes forward the leading edge of motile eukaryotic cells and intracellular…”
Get full text

EPLIN Regulates Actin Dynamics by Cross-Linking and Stabilizing Filaments by Maul, Raymond S., Song, Yuhong, Amann, Kurt J., Gerbin, Sachi C., Pollard, Thomas D., Chang, David D.

“…Epithelial protein lost in neoplasm (EPLIN) is a cytoskeleton-associated protein encoded by a gene that is down-regulated in transformed cells. EPLIN increases…”
Get full text

Structure of Arp2/3 Complex in Its Activated State and in Actin Filament Branch Junctions by Volkmann, Niels, Amann, Kurt J., Stoilova-McPhie, Svetla, Egile, Coumaran, Winter, Dirk C., Hazelwood, Larnele, Heuser, John E., Li, Rong, Pollard, Thomas D., Hanein, Dorit

“…The seven-subunit Arp2/3 complex choreographs the formation of branched actin networks at the leading edge of migrating cells. When activated by…”
Get full text

Polymerization Properties of the Thermotoga maritima Actin MreB:  Roles of Temperature, Nucleotides, and Ions by Bean, Greg J, Amann, Kurt J

“…MreB is a bacterial orthologue of actin that affects cell shape, polarity, and chromosome segregation. Although a significant body of work has explored its…”
Get full text

Proteolysis of cortactin by calpain regulates membrane protrusion during cell migration by Perrin, Benjamin J, Amann, Kurt J, Huttenlocher, Anna

“…Calpain 2 regulates membrane protrusion during cell migration. However, relevant substrates that mediate the effects of calpain on protrusion have not been…”
Get full text

A Cluster of Basic Repeats in the Dystrophin Rod Domain Binds F-actin through an Electrostatic Interaction by Amann, Kurt J., Renley, Brian A., Ervasti, James M.

“…The dystrophin rod domain is composed of 24 spectrin-like repeats and was thought to act mainly as a flexible spacer between the amino-terminal actin binding…”
Get full text

A New Model for the Interaction of Dystrophin with F-Actin by Rybakova, Inna N., Amann, Kurt J., Ervasti, James M.

“…The F-actin binding and cross-linking properties of skeletal muscle dystrophin-glycoprotein complex were examined using high and low speed cosedimentation…”
Get full text

Assembly properties of the Bacillus subtilis actin, MreB by Mayer, Joshua A., Amann, Kurt J.

“…The bacterial actin MreB has been implicated in a variety of cellular roles including cell shape determination, cell wall synthesis, chromosome condensation…”
Get full text

Utrophin Lacks the Rod Domain Actin Binding Activity of Dystrophin by Amann, K J, Guo, A W, Ervasti, J M

“…We previously identified a cluster of basic spectrin-like repeats in the dystrophin rod domain that binds F-actin through electrostatic interactions (Amann, K…”
Get full text

Polymerization Properties of the Thermotoga maritima Actin MreB: Roles of Temperature, Nucleotides, and Ions by Bean, Greg J, Amann, Kurt J

“…MreB is a bacterial orthologue of actin that affects cell shape, polarity, and chromosome segregation. Although a significant body of work has explored its…”
Get full text

POLYMERIZATION PROPERTIES OF THE T. MARITIMA ACTIN, MreB: ROLES OF TEMPERATURE, NUCLEOTIDES AND IONS by Bean, Greg J., Amann, Kurt J.

“…MreB is a bacterial ortholog of actin that affects cell shape, polarity and chromosome segregation. Although a significant body of work has explored its…”
Get full text

Proteolysis of Cortactin by Calpain Regulates Membrane Protrusion during Cell MigrationD by Perrin, Benjamin J., Amann, Kurt J., Huttenlocher, Anna

“…Calpain 2 regulates membrane protrusion during cell migration. However, relevant substrates that mediate the effects of calpain on protrusion have not been…”
Get full text

Cellular regulation of actin network assembly by Amann, Kurt J., Pollard, Thomas D.

Get full text

Dystrophin binding to nonmuscle actin by Renley, Brian A., Rybakova, Inna N., Amann, Kurt J., Ervasti, James M.

“…We purified actin from bovine brain by DNase I affinity chromatography in order to compare the binding of dystrophin to muscle actin with its binding to…”
Get full text