Antimicrobial susceptibility phenotypes, resistance determinants and DNA fingerprints of Salmonella enterica serotype Typhimurium isolated from bovine in Southern Japan

Antimicrobial susceptibility phenotypes, resistance determinants and DNA fingerprints of Salmonella enterica serotype Typhimurium isolated from bovine in Southern Japan

Abstract A longitudinal study was conducted in cattle to determine the antimicrobial resistance phenotypes, integron elements, resistance genes and pulsed-field gel electrophoresis fingerprints among Salmonella enterica serotype Typhimurium isolates. A total of 33 strains were isolated and categoris...

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Bibliographic Details
Published in:International journal of antimicrobial agents Vol. 30; no. 2; pp. 150 - 156
Main Authors: Shahada, Francis, Amamoto, Asako, Chuma, Takehisa, Shirai, Akito, Okamoto, Karoku
Format: Journal Article
Language:English
Published: London Elsevier B.V 01-08-2007
Amsterdam Elsevier
New York, NY
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antimicrobial resistance
Biological and medical sciences
Cattle
DNA Fingerprinting
Drug Resistance, Multiple, Bacterial
Infectious Disease
Integrons - genetics
Japan - epidemiology
Longitudinal Studies
Medical sciences
Microbial Sensitivity Tests
Pharmacology. Drug treatments
Salmonella enterica
Salmonella Infections, Animal - epidemiology
Salmonella Infections, Animal - microbiology
Salmonella Typhimurium
Salmonella typhimurium - drug effects
Salmonella typhimurium - genetics
Salmonella typhimurium - isolation & purification
Trends
Asia
Japan
Antimicrobial resistance
Trends
Cattle
Salmonella Typhimurium
Typing
Bovine
Drug susceptibility test
Ox
Salmonella typhimurium
Antimicrobial agent
Resistance
Vertebrata
Phenotype
Mammalia
Sensitivity resistance
Animal
DNA
Fingerprint method
Bacteria
Artiodactyla
Serotype
Veterinary
Antibacterial agent
Ungulata
Enterobacteriaceae
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Summary:Abstract A longitudinal study was conducted in cattle to determine the antimicrobial resistance phenotypes, integron elements, resistance genes and pulsed-field gel electrophoresis fingerprints among Salmonella enterica serotype Typhimurium isolates. A total of 33 strains were isolated and categorised into Groups A, B and C during the period 1989–2004. Thirty-one strains (93.9%) showed resistance to ampicillin (A) encoded by blaOXA-1 , blaTEM and blaPSE-1 genes; 84.8% showed resistance to chloramphenicol (C) encoded by floR and catA1 ; 97.0% were resistant both to streptomycin (S) and sulfamethoxazole (Su), the former encoded by aadA1 and aadA2 ; 100% were resistant to oxytetracycline (T) encoded by tetA , tetB and tetG ; and 42.4% were resistant to kanamycin (Km) encoded by aphA1-Iab . Multidrug resistance types observed were ACSSuT-Km ( n = 13), ACSSuT ( n = 15), ASSuT ( n = 3) and SSuT ( n = 2). Class 1 integrons ranging from 1.0 kb to 1.9 kb were detected from 54.5% of isolates (18/33). Integrons were not detected initially (1989–1992), then during the 1993–1996 interval a high frequency of 1.0 kb and 1.2 kb amplicons were detected and during 2000–2004 the amplicon size increased to 1.7 kb and 1.9 kb. We report evidence of additional integration of resistance gene cassettes as shown by integrons with increased size. Finally, group B strains showed banding patterns indistinguishable from S. Typhimurium DT104 reference strain, indicating that the DT104 lineage existed on the island since 1993.
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ISSN:0924-8579
1872-7913
DOI:10.1016/j.ijantimicag.2007.03.017