Detection of Vancomycin Resistant Genes in Intrinsically Antibiotic Resistant Bacteria from the Gut Microbiota of Indonesian Individuals

Detection of Vancomycin Resistant Genes in Intrinsically Antibiotic Resistant Bacteria from the Gut Microbiota of Indonesian Individuals

Antibiotic resistance is a global public health concern that has been exacerbated by the overuse and misuse of antibiotics, leading to the emergence of resistant bacteria. The gut microbiota, often influenced by antibiotic usage, plays a crucial role in overall health. Therefore, this study aimed to...

Full description

Saved in:
Bibliographic Details
Published in:Iranian journal of medical sciences Vol. 49; no. 5; pp. 302 - 312
Main Authors: Luqman, Arif, Saising, Jongkon, Prasetya, Yulianto Ade, Ammanath, Aparna Viswanathan, Andini, Amala, Siti Nur, Zulaika, Enny, Kuswytasari, Nengah Dwianita, Goetz, Friedrich, Wibowo, Anjar Tri
Format: Journal Article
Language:English
Published: Iran Shiraz University of Medical Sciences 01-05-2024
Subjects:
Adult
Analysis
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Antibiotics
Bacteria
Bacteria - classification
Bacteria - drug effects
Bacteria - genetics
Drug resistance in microorganisms
Feces - microbiology
Female
gastrointestinal microbiome
Gastrointestinal Microbiome - drug effects
Genes
Genes, Bacterial
health-risk
Humans
Indonesia
Male
Methicillin
Microbiota
Microbiota (Symbiotic organisms)
Original
Public health
Sulfonamides
Tetracycline
Tetracyclines
Vancomycin
Vancomycin - pharmacology
Vancomycin - therapeutic use
vancomycin resistance
Vancomycin Resistance - genetics
Indonesia
Germany
Health-risk
Antibiotics
Gastrointestinal microbiome
Vancomycin resistance
Indonesia
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Antibiotic resistance is a global public health concern that has been exacerbated by the overuse and misuse of antibiotics, leading to the emergence of resistant bacteria. The gut microbiota, often influenced by antibiotic usage, plays a crucial role in overall health. Therefore, this study aimed to investigate the prevalence of antibiotic resistant genes in the gut microbiota of Indonesian coastal and highland populations, as well as to identify vancomycin-resistant bacteria and their resistant genes. Stool samples were collected from 22 individuals residing in Pacet, Mojokerto, and Kenjeran, Surabaya Indonesia in 2022. The read count of antibiotic resistant genes was analyzed in the collected samples, and the bacterium concentration was counted by plating on the antibiotic-containing agar plate. Vancomycin-resistant strains were further isolated, and the presence of vancomycin-resistant genes was detected using a multiplex polymerase chain reaction (PCR). The antibiotic resistant genes for tetracycline, aminoglycosides, macrolides, beta-lactams, and vancomycin were found in high frequency in all stool samples (100%) of the gut microbiota. Meanwhile, those meant for chloramphenicol and sulfonamides were found in 86% and 16% of the samples, respectively. Notably, vancomycin-resistant genes were found in 16 intrinsically resistant Gram-negative bacterial strains. Among the detected vancomycin-resistant genes, was the most prevalent (27.3%), while was the least prevalent (4.5%). The presence of multiple vancomycin resistance genes in intrinsically resistant Gram-negative bacterial strains demonstrated the importance of the gut microbiota as a reservoir and hub for the horizontal transfer of antibiotic resistant genes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0253-0716
1735-3688
DOI:10.30476/ijms.2023.98767.3087