High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry

A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2386; p. 43
Main Authors:	Fernandez, Marco A, Alzayat, Hammad, Jaimes, Maria C, Kharraz, Yacine, Requena, Gerard, Mendez, Pedro
Format:	Journal Article
Language:	English
Published:	United States 2022
Subjects:	Color COVID-19 - blood Flow Cytometry Humans Immune System Immunophenotyping - methods Deep immunophenotyping Full-spectrum cytometry Spectral signature
Online Access:	Get more information
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Summary:	A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, we describe a 37-color protocol that allows to identify more than 45 different cell populations on whole blood samples of SARS-CoV-2-infected patients.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-1771-7_4