Antidiarrheal effect of extract from the bark of Combretum leprosum in mice

This study investigated the effects of the ethanolic extract from the bark of Combretum leprosum (ECL) on intestinal transit and castor-oil induced diarrhea in mice. The oral administration of ECL (750 and 1000 mg/kg) slowed intestinal transit (ID50 of 455 mg/kg). The ECL (250-1000 mg/kg) reduced ca...

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Bibliographic Details
Published in:	Anais da Academia Brasileira de Ciências Vol. 91; no. 1; p. e20170932
Main Authors:	Cavalcanti, Paulo M S, Martins, Maria DO Carmo C, Nunes, Paulo H M, Alves Filho, Francisco C, Silva, Janyerson D P, Cavalcanti, Suzana M G
Format:	Journal Article
Language:	English
Published:	Brazil Academia Brasileira de Ciências 01-01-2019
Subjects:	antidiarrheal effect Combretum leprosum enteropooling intestinal transit medicinal plants MULTIDISCIPLINARY SCIENCES polyphenols enteropooling polyphenols antidiarrheal effect medicinal plants Combretum leprosum intestinal transit
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Summary:	This study investigated the effects of the ethanolic extract from the bark of Combretum leprosum (ECL) on intestinal transit and castor-oil induced diarrhea in mice. The oral administration of ECL (750 and 1000 mg/kg) slowed intestinal transit (ID50 of 455 mg/kg). The ECL (250-1000 mg/kg) reduced castor-oil induced diarrhea, in a time- and dose-dependent manner (p < 0.05). To determine if antidiarrheal effect of ECL involves α2-adrenergic or opioid receptor activation, the mice were pretreated with antagonists of these receptors, yohimbine or naloxone respectively. None of these drugs inhibited the antidiarrheal effect of ECL. To test if antidiarrheal effect of ECL is due to an antisecretory action, we realized the enteropooling assay on rats. The ECL increased bowel content and did not inhibit intestinal fluid secretion increase induced by misoprostol (100 µg/kg, s.c.). To determine if antimotility effect of ECL is due to a reduction on gastric motility, we realized the organ bath assay in the rat fundus stomach. Isotonic recordings show that the carbachol /KCl - induced contraction was not reversed by the addition of ECL. In conclusion, our results suggest that ECL contains antidiarrheal compounds and these compounds could induce a reduction of intestinal tract motility.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0001-3765 1678-2690 1678-2690
DOI:	10.1590/0001-3765201820170932