Development of a new biomaterial based on cashew tree gum (Anarcadium occidentale L.) enriched with hydroxyapatite and evaluation of cytotoxicity in adipose-derived stem cell cultures

Development of a new biomaterial based on cashew tree gum (Anarcadium occidentale L.) enriched with hydroxyapatite and evaluation of cytotoxicity in adipose-derived stem cell cultures

Cashew tree gum is a polysaccharide material highly available in the Northeast region of Brazil. It has been explored for biocompatibility with human tissues. This research aimed to describe the synthesis and characterization of cashew gum/hydroxyapatite scaffold and evaluate the possible cytotoxici...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules Vol. 242; no. Pt 2; p. 124864
Main Authors: da Rocha, Lorenna Bastos Nogueira, Sousa, Ricardo Barbosa, dos Santos, Marcus Vinicius Beserra, Argolo Neto, Napoleão Martins, Soares, Letícya Lorrayne da Silva, Alves, Francisca Louenny Cardoso, de Carvalho, Maria Acelina Martins, Osajima, Josy Anteveli, Silva-Filho, Edson C.
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 01-07-2023
Subjects:
Anacardium
Animals
Apatites
Biocompatible Materials - pharmacology
Bone repair
Cell Culture Techniques
Cell Differentiation
Durapatite
Humans
Mice
Osteogenesis
Polysaccharides
Rats
Rats, Wistar
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Trees
Bone repair
Polysaccharides
Apatites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cashew tree gum is a polysaccharide material highly available in the Northeast region of Brazil. It has been explored for biocompatibility with human tissues. This research aimed to describe the synthesis and characterization of cashew gum/hydroxyapatite scaffold and evaluate the possible cytotoxicity in murine adipose-derived stem cells (ADSCs) cultures. ADSCs of the subcutaneous fat tissue of Wistar rats were collected, isolated, expanded, differentiated into three strains, and characterized immunophenotypically. The scaffolds were synthesized through chemical precipitation, lyophilized and characterized through scanning electron microscopy (SEM), infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermal analysis (TG and DTG), and mechanical testing. The scaffold presented a crystalline structure and pores with an average diameter of 94.45 ± 50.57 μm. By mechanical tests, the compressive force and modulus of elasticity were like the cancellous bone. The isolated adipose-derived stem cells (ADSCs) presented fibroblast morphology, adhesion capacity to plastic, differentiation in osteogenic, adipogenic and chondrogenic lineages, positive expression for the CD105 and CD90 markers and negative expression for the CD45 and CD14 markers. The MTT test showed increased cell viability, and the biomaterial showed a high level of hemocompatibility (<5 %). This study allowed the development of a new scaffold for future surgical applicability in tissue regeneration.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.124864