The pan-genome of the emerging multidrug-resistant pathogen Corynebacterium striatum

Corynebacterium striatum , a common constituent of the human skin microbiome, is now considered an emerging multidrug-resistant pathogen of immunocompromised and chronically ill patients. However, little is known about the molecular mechanisms in the transition from colonization to the multidrug-res...

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Published in:	Functional & integrative genomics Vol. 23; no. 1; p. 5
Main Authors:	Jesus, Hendor N. R., Ramos, Juliana N., Rocha, Danilo J. P. G., Alves, Daniele A., Silva, Carolina S., Cruz, João V. O., Vieira, Verônica V., Souza, Cassius, Santos, Louisy S., Navas, Jesus, Ramos, Rommel T. J., Azevedo, Vasco, Aguiar, Eric R. G. R., Mattos-Guaraldi, Ana L., Pacheco, Luis G. C.
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2023 Springer Nature B.V
Subjects:	Animal Genetics and Genomics Anti-Bacterial Agents Antimicrobial resistance Biochemistry Bioinformatics Biomedical and Life Sciences Cell Biology Clinical isolates Corynebacterium Corynebacterium striatum Drug Resistance, Multiple, Bacterial - genetics Gene families Genetic factors Genomes Genomic analysis Genomic islands Humans Life Sciences Microbial Genetics and Genomics Microbial Sensitivity Tests Microbiomes Molecular modelling Multidrug resistance Multidrug resistant organisms Original Article Pathogenicity Pathogens Phenotype Phenotypes Phenotypic plasticity Plant Genetics and Genomics Tetracyclines Virulence Virulence factors Virulence Factors - genetics Pan-genome Multidrug resistance Emerging pathogen Corynebacterium striatum
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Summary:	Corynebacterium striatum , a common constituent of the human skin microbiome, is now considered an emerging multidrug-resistant pathogen of immunocompromised and chronically ill patients. However, little is known about the molecular mechanisms in the transition from colonization to the multidrug-resistant (MDR) invasive phenotype in clinical isolates. This study performed a comprehensive pan-genomic analysis of C. striatum , including isolates from “normal skin microbiome” and from MDR infections, to gain insights into genetic factors contributing to pathogenicity and multidrug resistance in this species. For this, three novel genome sequences were obtained from clinical isolates of C. striatum of patients from Brazil, and other 24 complete or draft C. striatum genomes were retrieved from GenBank, including the ATCC6940 isolate from the Human Microbiome Project. Analysis of C. striatum strains demonstrated the presence of an open pan-genome (α = 0.852803) containing 3816 gene families, including 15 antimicrobial resistance (AMR) genes and 32 putative virulence factors. The core and accessory genomes included 1297 and 1307 genes, respectively. The identified AMR genes are primarily associated with resistance to aminoglycosides and tetracyclines. Of these, 66.6% are present in genomic islands, and four AMR genes, including aac(6')-ib7 , are located in a class 1-integron. In conclusion, our data indicated that C. striatum possesses genomic characteristics favorable to the invasive phenotype, with high genomic plasticity, a robust genetic arsenal for iron acquisition, and important virulence determinants and AMR genes present in mobile genetic elements.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1438-793X 1438-7948
DOI:	10.1007/s10142-022-00932-x