Search Results - "Alves, Christina S"

Integrated cell and process engineering for improved transient production of a "difficult-to-express" fusion protein by CHO cells by Johari, Yusuf B., Estes, Scott D., Alves, Christina S., Sinacore, Marty S., James, David C.

“…ABSTRACT Based on an optimized electroporation protocol, we designed a rapid, milliliter‐scale diagnostic transient production assay to identify limitations in…”
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Carcinoembryonic Antigen and CD44 Variant Isoforms Cooperate to Mediate Colon Carcinoma Cell Adhesion to E- and L-selectin in Shear Flow by Thomas, Susan N., Zhu, Fei, Schnaar, Ronald L., Alves, Christina S., Konstantopoulos, Konstantinos

“…Selectin-mediated adhesion of tumor cells to platelets, leukocytes, and endothelial cells may regulate their hematogenous dissemination in the…”
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Distinct Kinetic and Molecular Requirements Govern CD44 Binding to Hyaluronan versus Fibrin(ogen) by Raman, Phrabha S., Alves, Christina S., Wirtz, Denis, Konstantopoulos, Konstantinos

“…CD44 is a multifunctional glycoprotein that binds to hyaluronan and fibrin(ogen). Alternative splicing is responsible for the generation of numerous different…”
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CHOmics: A web-based tool for multi-omics data analysis and interactive visualization in CHO cell lines by Lin, Dongdong, Yalamanchili, Hima B, Zhang, Xinmin, Lewis, Nathan E, Alves, Christina S, Groot, Joost, Arnsdorf, Johnny, Bjørn, Sara P, Wulff, Tune, Voldborg, Bjørn G, Zhou, Yizhou, Zhang, Baohong

“…Chinese hamster ovary (CHO) cell lines are widely used in industry for biological drug production. During cell culture development, considerable effort is…”
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PDGF suppresses the sulfation of CD44v and potentiates CD44v-mediated binding of colon carcinoma cells to fibrin under flow by Alves, Christina S, Konstantopoulos, Konstantinos

“…Fibrin(ogen) mediates sustained tumor cell adhesion and survival in the pulmonary vasculature, thereby facilitating the metastatic dissemination of tumor…”
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The dual role of CD44 as a functional P-selectin ligand and fibrin receptor in colon carcinoma cell adhesion by Alves, Christina S, Burdick, Monica M, Thomas, Susan N, Pawar, Parag, Konstantopoulos, Konstantinos

“…Selectins and fibrin(ogen) play key roles in the hematogenous dissemination of tumor cells, and especially of colon carcinomas. However, the fibrin(ogen)…”
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Strategies and Considerations for Improving Expression of "Difficult to Express" Proteins in CHO Cells by Alves, Christina S, Dobrowsky, Terrence M

“…Despite substantial advances in the field of mammalian expression, there are still proteins that are characterized as difficult to express. Determining the…”
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CHO genome mining for synthetic promoter design by Johari, Yusuf B., Brown, Adam J., Alves, Christina S., Zhou, Yizhou, Wright, Chapman M., Estes, Scott D., Kshirsagar, Rashmi, James, David C.

“…•Bioinformatic analysis of endogenous CHO promoter sequences.•Linking transcriptomic and genomic datasets for bioprocess-directed promoter design.•In silico…”
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Single-molecule binding of CD44 to fibrin versus P-selectin predicts their distinct shear-dependent interactions in cancer by Raman, Phrabha S, Alves, Christina S, Wirtz, Denis, Konstantopoulos, Konstantinos

“…P-selectin and fibrin(ogen) have pivotal roles in the hematogenous dissemination of tumor cells. CD44 variant isoforms, CD44v, have been identified as the…”
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Biomolecular characterization of CD44-fibrin(ogen) binding: distinct molecular requirements mediate binding of standard and variant isoforms of CD44 to immobilized fibrin(ogen) by Alves, Christina S, Yakovlev, Sergiy, Medved, Leonid, Konstantopoulos, Konstantinos

“…CD44 and fibrin(ogen) play critical roles in the hematogenous dissemination of tumor cells, including colon carcinomas. We recently reported that CD44 is the…”
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Integration of cell line and process development to overcome the challenge of a difficult to express protein by Alves, Christina S., Gilbert, Alan, Dalvi, Swati, Germain, Bryan St, Xie, Wenqi, Estes, Scott, Kshirsagar, Rashmi, Ryll, Thomas

“…This case study addresses the difficulty in achieving high level expression and production of a small, very positively charged recombinant protein. The novel…”
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Biomolecular Characterization of CD44-Fibrin(ogen) Binding by Alves, Christina S., Yakovlev, Sergiy, Medved, Leonid, Konstantopoulos, Konstantinos

“…CD44 and fibrin(ogen) play critical roles in the hematogenous dissemination of tumor cells, including colon carcinomas. We recently reported that CD44 is the…”
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Defective blood vessel development and pericyte/pvSMC distribution in α4 integrin-deficient mouse embryos by Grazioli, Alison, Alves, Christina S., Konstantopoulos, Konstantinos, Yang, Joy T.

“…Blood vessel development is in part regulated by pericytes/presumptive vascular smooth muscle cells (PC/pvSMCs). Here, we demonstrate that interactions between…”
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Single Molecule Binding of CD44 to Fibrin Versus P-selectin Predicts Their Distinct Shear-dependent Interactions in Cancer by Raman, Phrabha S., Alves, Christina S., Wirtz, Denis, Konstantopoulos, Konstantinos

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Defective blood vessel development and pericyte/pvSMC distribution in alpha 4 integrin-deficient mouse embryos by Grazioli, Alison, Alves, Christina S, Konstantopoulos, Konstantinos, Yang, Joy T

“…Blood vessel development is in part regulated by pericytes/presumptive vascular smooth muscle cells (PC/pvSMCs). Here, we demonstrate that interactions between…”
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alpha4 beta1-Integrin regulates directionally persistent cell migration in response to shear flow stimulation by Dikeman, Dustin A, Rivera Rosado, Leslie A, Horn, Troy A, Alves, Christina S, Konstantopoulos, Konstantinos, Yang, Joy T

“…alpha(4)beta(1)-Integrin plays a pivotal role in cell migration in vivo. This integrin has been shown to regulate the front-back polarity of migrating cells…”
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Fibrin(ogen) is a CD44 ligand on colon carcinoma cells: Biochemical and biophysical characterization, growth factor involvement, and diagnostic applications by Alves, Christina S

“…Selectins, CD44, and fibrin(ogen) play critical roles in the hematogenous dissemination of tumor cells, including colon carcinomas. However, the fibrin(ogen)…”
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Fibrin(ogen) is a CD44 ligand on colon carcinoma cells: Biochemical and biophysical characterization, growth factor involvement, and diagnostic applications by Alves, Christina S

“…Selectins, CD44, and fibrin(ogen) play critical roles in the hematogenous dissemination of tumor cells, including colon carcinomas. However, the fibrin(ogen)…”
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