Natural antioxidants in the vitrification solution improve the ovine ovarian tissue preservation

Natural antioxidants in the vitrification solution improve the ovine ovarian tissue preservation

The present study evaluated the effect of the addition of antioxidants anethole (AN) and robinin (RO) in the vitrification solution, and the in vitro incubation (IVI) medium of ovine ovarian tissue. Ovarian fragments were vitrified without antioxidant (VWA) or with different concentrations of AN (30...

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Bibliographic Details
Published in:Reproductive biology Vol. 19; no. 3; pp. 270 - 278
Main Authors: Dos Santos Morais, Maria Luana Gaudencio, de Brito, Danielle Cristina Calado, Pinto, Yago, Mascena Silva, Luciana, Montano Vizcarra, Diego, Silva, Renato Félix, Weber Santos Cibin, Franciele, Cabral Campello, Claúdio, Alves, Benner Gerardo, Rocha Araújo, Valdevane, da Chagas Pinto, Francisco, Pessoa, Otília Deusdênia Loiola, Figueiredo, José Ricardo, Ribeiro Rodrigues, Ana Paula
Format: Journal Article
Language:English
Published: Poland 01-09-2019
Subjects:
Animals
Antioxidants - metabolism
Cryopreservation - methods
Cryopreservation - veterinary
Culture Media
Female
Ovary - drug effects
Reactive Oxygen Species - metabolism
Sheep
Tissue Preservation - veterinary
Vitrification
Robinin
Preantral
Anethole
Follicles
Cryopreservation
ROS
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Summary:The present study evaluated the effect of the addition of antioxidants anethole (AN) and robinin (RO) in the vitrification solution, and the in vitro incubation (IVI) medium of ovine ovarian tissue. Ovarian fragments were vitrified without antioxidant (VWA) or with different concentrations of AN (30, 300 and 2000 μg/mL) or RO (0.125, 0.25 and 0.50 mg/mL), followed by IVI (24 h). Histological analyses showed that the percentage of morphologically normal preantral follicles (MNPF) in AN 2000 did not differ from RO 0.125 or fresh ovarian tissue (CTR). Subsequently, ovarian fragments were vitrified in the presence of AN 2000 and RO 0.125 followed by IVI without or with (AN 2000+ and RO 0.125+) the same antioxidants. The follicular activation in all treatments was significantly increased as compared to the CTR. The stroma cell density (SCD) in all the vitrified fragments was significantly lower than the CTR. However, in the AN 2000 and RO 0.125 this parameter was significantly higher when compared to the VWA. The reactive oxygen species (ROS) in the ovarian cortex of the AN 2000 or AN 2000+ were significantly reduced in comparison with the CTR while the intracellular ROS levels of AN 2000 and CTR were similar. The total antioxidant capacity (TAC) in RO 0.125 was significantly higher than that of VWA, AN 2000 and AN 2000+. According to the results, the use of antioxidants (AN or RO) only in the vitrification solution of ovine ovarian tissue is recommended, due to their better preservation of the SCD. Moreover, AN 2000 best maintains the follicular morphology, while RO 0.125 has a high TAC.
ISSN:1642-431X
2300-732X
DOI:10.1016/j.repbio.2019.07.008