Predatory effects of the fungus Arthrobotrys cladodes on sheep gastrointestinal nematodes

The biological control of gastrointestinal nematodes of animals using nematophagous fungi is considered a promising approach. The objective of this study was to evaluate the effectiveness of Arthrobotrys cladodes (CG 719) isolates in vitro and after passage through the gastrointestinal tract of shee...

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Bibliographic Details
Published in:	Biocontrol science and technology Vol. 30; no. 8; pp. 830 - 839
Main Authors:	Rodrigues, Jossiara Abrante, Alvares, Felipe Bondiej Ventura, Silva, Juliana Trajano, Ferreira, Larissa Claudino, Lopes da Costa, Paulo Wbiratan, Sarmento, Wlysse Ferreira, Ferreira Feitosa, Thais, Araújo, Jackson Victor de, Braga, Fabio Ribeiro, Longo Ribeiro Vilela, Vinicius
Format:	Journal Article
Language:	English
Published:	Abingdon Taylor & Francis 02-08-2020 Taylor & Francis Ltd
Subjects:	Animals Assaying Biological control Experiments Fungi Gastrointestinal system Gastrointestinal tract Inoculation Intestinal parasites Larvae Nematodes Nematophagous fungi Sheep sheep helminthiasis
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Summary:	The biological control of gastrointestinal nematodes of animals using nematophagous fungi is considered a promising approach. The objective of this study was to evaluate the effectiveness of Arthrobotrys cladodes (CG 719) isolates in vitro and after passage through the gastrointestinal tract of sheep. In experiment 1, the in vitro predatory capacity of A. cladodes against gastrointestinal nematodes in sheep was evaluated. Petri dishes containing fungi cultured on water agar medium were inoculated with 2,000 infective larvae of sheep gastrointestinal nematodes. Experiment 2 involved treatment and control groups comprising six sheep each. The animals in the treatment group received single 10 g doses of pellets containing fungal formulations, and fecal samples were collected after 12, 24, 36, 48, 60, and 72 h. In experiment 1, a larval reduction of 72.3% was observed at 7 days after the larval inoculation. In both assays of experiment 2, larval reductions were significant (p < 0.01). In assay A, this fungus was able to pass throught the gastrointestinal tract of sheep, with a larval reduction of 83.5%. In assay B, proportion of larvae recovered from coprocultures was reduced by 72.4%. Thus, we concluded that pelleted formulations of A. cladodes were effective against gastrointestinal nematodes in vitro and after passage through the gastrointestinal tract of sheep.
ISSN:	0958-3157 1360-0478
DOI:	10.1080/09583157.2020.1775176