Identification of DLK1 variants in pituitary- and neuroendocrine tumors

In a gene chip analysis of common pituitary tumor types, one of the genes with the most impressive tissue-specific expression regulation was delta-like 1 (DLK1), which was strongly expressed in GH-secreting (GH-S) pituitary tumors. In addition to pituitary adenomas, various endocrine tumors were sub...

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Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 340; no. 3; pp. 995 - 1005
Main Authors:	Altenberger, T., Bilban, M., Auer, M., Knosp, E., Wolfsberger, S., Gartner, W., Mineva, I., Zielinski, C., Wagner, L., Luger, A.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 17-02-2006
Subjects:	Alternative Splicing Base Sequence Blotting, Northern Cloning, Molecular Codon, Terminator DLK1 DNA - chemistry DNA - metabolism DNA, Complementary - metabolism Female Gene Expression Profiling Gene Expression Regulation Gene Expression Regulation, Neoplastic Genetic Variation Humans Immunoblotting Intercellular Signaling Peptides and Proteins Male Membrane Proteins - biosynthesis Membrane Proteins - chemistry Membrane Proteins - metabolism Microscopy, Confocal Microscopy, Fluorescence Models, Genetic Molecular Sequence Data Neuroendocrine tumors Neuroendocrine Tumors - metabolism Oligonucleotide Array Sequence Analysis Ovarian Neoplasms - metabolism Pituitary Pituitary adenomas Pituitary Neoplasms - metabolism Polymerase Chain Reaction Pref-1 Protein Structure, Tertiary Recombinant Proteins - chemistry Repressor Proteins - biosynthesis Repressor Proteins - chemistry Repressor Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Sequence Homology, Nucleic Acid Time Factors Transcript variants Pituitary adenomas Transcript variants Pituitary DLK1 Pref-1 Neuroendocrine tumors
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Summary:	In a gene chip analysis of common pituitary tumor types, one of the genes with the most impressive tissue-specific expression regulation was delta-like 1 (DLK1), which was strongly expressed in GH-secreting (GH-S) pituitary tumors. In addition to pituitary adenomas, various endocrine tumors were subjected to real-time-quantitative PCR revealing high expression of DLK1 in normal pituitary tissue, in GH-S-, in one prolactin-secreting pituitary adenoma and in pheochromocytomas. Additionally, three DLK1 gene-derived subvariants were identified. The first, lacking 204 bp—coding for epidermal growth factor-like domain 6 and parts of the juxtamembrane region—was named Secredeltin. In the other two splice variants (named Brevideltin and Brevideltinin), a stop codon is introduced due to a frame-shift, leading to truncated proteins of 204 and 213 aas, respectively.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-291X 1090-2104
DOI:	10.1016/j.bbrc.2005.12.094