Molecular Phylogenetics of Physa acuta (Pulmonata: Basommatophora): an Invasive species in Central Punjab Pakistan

Molecular Phylogenetics of Physa acuta (Pulmonata: Basommatophora): an Invasive species in Central Punjab Pakistan

Abstract Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were...

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Published in:Brazilian journal of biology Vol. 83; pp. 1 - 8
Main Authors: Ansari, B., Altafa, J., Ramzan, A., Ahmed, Z., Khalil, S., Qamar, S. U. R., Awan, S. A., Jehangir, K., Khalid, R., Aziz, S., Sultana, T., Sultana, S., Alsamadany, H., Alshamrani, R., Awan, F. S.
Format: Journal Article
Language:English
Published: São Carlos Instituto Internacional de Ecologia 2023
Subjects:
Amplification
Basommatophora
Bioindicators
BIOLOGY
Canals
Cloning
DNA polymerase
Gastropoda
Genetic diversity
Genetic testing
Indicator species
Indigenous species
Introduced species
Invasive species
Markers
molecular marker
Mollusca
Mollusks
Multivariate analysis
Native species
Nonnative species
Parasites
Pests
Phylogenetics
Phylogeny
Physa
Physa acuta
Physa gyrina
Polymorphism
Pulmonata
Random amplified polymorphic DNA
Punjab Pakistan
United States > US
Pakistan
genetic diversity
physa
polimorfismo
mollusca
molecular marker
diversidade genética
polymorphism
marcador molecular
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Summary:Abstract Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were characterized on the basis of molecular markers High level of genetic diversity was revealed by polymorphic RAPD, however SSR markers were not amplified. The multivariate analysis revealed polymorphism ranging from 9.09 percent to 50 percent among the three Physid species. Total number of 79 loci were observed for the three species under study and 24 loci were observed to be polymorphic. These RAPD fragment(s) can be developed into co dominant markers (SCAR) by cloning and can be further sequenced for the development of the Physa species specific markers to identify the introduced and native species in Pakistan. Resumo Os físidos pertencem à classe Gastropoda; pertencem ao filo Mollusca e, sendo bioindicadores, hospedeiros intermediários de parasitas e pragas, ocupam uma posição-chave no ecossistema. Existem três espécies do gênero Physa, ou seja, P. fontinalis, Physa acuta e P. gyrina em corpos d’água do Punjab Central e foram caracterizadas com base em marcadores moleculares. Alto nível de diversidade genética foi revelado por RAPD polimórfico, no entanto os marcadores SSR não foram amplificados. A análise multivariada revelou polimorfismo variando de 9,09% a 50% entre as três espécies de Physid. Um número total de 79 loci foi observado para as três espécies em estudo e 24 loci foram observados como polimórficos. Esses fragmentos RAPD podem ser desenvolvidos em marcadores codominantes (SCAR) por clonagem e podem ser posteriormente sequenciados para o desenvolvimento de marcadores específicos da espécie Physa para identificar as espécies introduzidas e nativas no Paquistão.
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ISSN:1519-6984
1678-4375
1678-4375
DOI:10.1590/1519-6984.246984