The development of an electrochemical immunosensor utilizing chicken IgY anti-spike antibody for the detection of SARS-CoV-2

This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA...

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Bibliographic Details
Published in:	Scientific reports Vol. 14; no. 1; p. 748
Main Authors:	Al-Qaoud, Khaled M., Obeidat, Yusra M., Al-Omari, Tareq, Okour, Mohammad, Al-Omari, Mariam M., Ahmad, Mohammad I., Alshadfan, Raed, Rawashdeh, AbdelMonem M.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 07-01-2024 Nature Publishing Group Nature Portfolio
Subjects:	631/1647 631/61 692/699 Animals Antibodies Antigens Biosensing Techniques Biosensors Blood Group Antigens Chickens COVID-19 COVID-19 - diagnosis Eggs Electric properties Electrochemistry Electrodes Enzyme-linked immunosorbent assay Humanities and Social Sciences Immunization Immunoassay Immunosensors Medical research multidisciplinary Proteins RNA, Viral SARS-CoV-2 Science Science (multidisciplinary) Severe acute respiratory syndrome coronavirus 2 Spike protein
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Summary:	This paper introduces a novel approach for detecting the SARS-CoV-2 recombinant spike protein combining a label free electrochemical impedimetric immunosensor with the use of purified chicken IgY antibodies. The sensor employs three electrodes and is functionalized with an anti-S IgY antibody, ELISA and immunoblot assays confirmed the positive response of chicken immunized with SARS-CoV2 S antigen. The developed immunosensor is effective in detecting SARS-CoV-2 in nasopharyngeal clinical samples from suspected cases. The key advantage of this biosensor is its remarkable sensitivity, and its capability of detecting very low concentrations of the target analyte, with a detection limit of 5.65 pg/mL. This attribute makes it highly suitable for practical point-of-care (POC) applications, particularly in low analyte count clinical scenarios, without requiring amplification. Furthermore, the biosensor has a wide dynamic range of detection, spanning from 11.56 to 740 ng/mL, which makes it applicable for sample analysis in a typical clinical setting.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-023-50501-w