Search Results - "Alphey, Magnus S."

Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation by Fisher, Gemma, Corbella, Marina, Alphey, Magnus S., Nicholson, John, Read, Benjamin J., Kamerlin, Shina C. L., da Silva, Rafael G.

“…ATP phosphoribosyltransferase catalyses the first step of histidine biosynthesis and is controlled via a complex allosteric mechanism where the regulatory…”
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Canonical binding of Chaetomium thermophilum DNA polymerase δ/ζ subunit PolD3 and flap endonuclease Fen1 to PCNA by Alphey, Magnus S, Wolford, Campbell B, MacNeill, Stuart A

“…The sliding clamp PCNA is a key player in eukaryotic genome replication and stability, acting as a platform onto which components of the DNA replication and…”
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Chemical, genetic and structural assessment of pyridoxal kinase as a drug target in the African trypanosome by Jones, Deuan C., Alphey, Magnus S., Wyllie, Susan, Fairlamb, Alan H.

“…Summary Pyridoxal‐5′‐phosphate (vitamin B6) is an essential cofactor for many important enzymatic reactions such as transamination and decarboxylation. African…”
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Structural and mechanistic insights into type II trypanosomatid tryparedoxin-dependent peroxidases by Alphey, Magnus S, König, Janine, Fairlamb, Alan H

“…TbTDPX (Trypanosoma brucei tryparedoxin-dependent peroxidase) is a genetically validated drug target in the fight against African sleeping sickness. Despite…”
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Non‐canonical binding of the Chaetomium thermophilum PolD4 N‐terminal PIP motif to PCNA involves Q‐pocket and compact 2‐fork plug interactions but no 310 helix by Yang, Dongxiao, Alphey, Magnus S., MacNeill, Stuart A.

“…DNA polymerase δ (Pol δ) is a key enzyme for the maintenance of genome integrity in eukaryotic cells, acting in concert with the sliding clamp processivity…”
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Non-canonical binding of the Chaetomium thermophilum PolD4 N-terminal PIP motif to PCNA involves Q-pocket and compact 2-fork plug interactions but no 3 10 helix by Yang, Dongxiao, Alphey, Magnus S, MacNeill, Stuart A

“…DNA polymerase δ (Pol δ) is a key enzyme for the maintenance of genome integrity in eukaryotic cells, acting in concert with the sliding clamp processivity…”
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The Synthesis of UDP-N-acetylglucosamine Is Essential for Bloodstream Form Trypanosoma brucei in Vitro and in Vivo and UDP-N-acetylglucosamine Starvation Reveals a Hierarchy in Parasite Protein Glycosylation by Stokes, Matthew J., Güther, M. Lucia S., Turnock, Daniel C., Prescott, Alan R., Martin, Kirstee L., Alphey, Magnus S., Ferguson, Michael A.J.

“…A gene encoding Trypanosoma brucei UDP-N-acetylglucosamine pyrophosphorylase was identified, and the recombinant protein was shown to have enzymatic activity…”
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Crystal Structures of Trypanosoma brucei and Staphylococcus aureus Mevalonate Diphosphate Decarboxylase Inform on the Determinants of Specificity and Reactivity by Byres, Emma, Alphey, Magnus S., Smith, Terry K., Hunter, William N.

“…Mevalonate diphosphate decarboxylase (MDD) catalyzes the ATP-dependent decarboxylation of mevalonate 5-diphosphate (MDP) to form isopentenyl pyrophosphate, a…”
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Biosynthesis of Isoprenoids: Crystal Structure of 4-Diphosphocytidyl-2C-Methyl-D-Erythritol Kinase by Miallau, Linda, Alphey, Magnus S., Kemp, Lauris E., Leonard, Gordon A., McSweeney, Sean M., Hecht, Stefan, Bacher, Adelbert, Eisenreich, Wolfgang, Rohdich, Felix, Hunter, William N.

“…4-Diphosphocytidyl-2C-methyl-D-erythritol kinase, an essential enzyme in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate…”
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Crystal Structure, Steady-State, and Pre-Steady-State Kinetics of Acinetobacter baumannii ATP Phosphoribosyltransferase by Read, Benjamin J., Cadzow, Andrew F., Alphey, Magnus S., Mitchell, John B. O., da Silva, Rafael G.

“…The first step of histidine biosynthesis in Acinetobacter baumannii, the condensation of ATP and 5-phospho-α-d-ribosyl-1-pyrophosphate to produce N…”
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Mapping the Structural Path for Allosteric Inhibition of a Short-Form ATP Phosphoribosyltransferase by Histidine by Thomson, Catherine M, Alphey, Magnus S, Fisher, Gemma, da Silva, Rafael G

“…ATP phosphoribosyltransferase (ATPPRT) catalyzes the first step of histidine biosynthesis, being allosterically inhibited by the final product of the pathway…”
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Next generation Glucose-1-phosphate thymidylyltransferase (RmlA) inhibitors: An extended SAR study to direct future design by Xiao, Ganyuan, Alphey, Magnus S., Tran, Fanny, Pirrie, Lisa, Milbeo, Pierre, Zhou, Yi, Bickel, Jasmine K., Kempf, Oxana, Kempf, Karl, Naismith, James H., Westwood, Nicholas J.

“…[Display omitted] The monosaccharide l-Rhamnose is an important component of bacterial cell walls. The first step in the l-rhamnose biosynthetic pathway is…”
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Synthesis and Characterization of Cytidine Derivatives that Inhibit the Kinase IspE of the Non-Mevalonate Pathway for Isoprenoid Biosynthesis by Crane, Christine M., Hirsch, Anna K. H., Alphey, Magnus S., Sgraja, Tanja, Lauw, Susan, Illarionova, Victoria, Rohdich, Felix, Eisenreich, Wolfgang, Hunter, William N., Bacher, Adelbert, Diederich, François

“…The enzymes of the non‐mevalonate pathway for isoprenoid biosynthesis are attractive targets for the development of novel drugs against malaria and…”
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Dihydroquinazolines as a Novel Class of Trypanosoma brucei Trypanothione Reductase Inhibitors: Discovery, Synthesis, and Characterization of their Binding Mode by Protein Crystallography by Patterson, Stephen, Alphey, Magnus S, Jones, Deuan C, Shanks, Emma J, Street, Ian P, Frearson, Julie A, Wyatt, Paul G, Gilbert, Ian H, Fairlamb, Alan H

“…Trypanothione reductase (TryR) is a genetically validated drug target in the parasite Trypanosoma brucei, the causative agent of human African trypanosomiasis…”
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Catalytic and Anticatalytic Snapshots of a Short-Form ATP Phosphoribosyltransferase by Alphey, Magnus S, Fisher, Gemma, Ge, Ying, Gould, Eoin R, Machado, Teresa F. G, Liu, Huanting, Florence, Gordon J, Naismith, James H, da Silva, Rafael G

“…Allosteric modulation of catalysis is a common regulatory strategy of flux-controlling biosynthetic enzymes. The enzyme ATP phosphoribosyltransferase (ATPPRT)…”
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Kinetics and Structure of a Cold-Adapted Hetero-Octameric ATP Phosphoribosyltransferase by Stroek, Rozanne, Ge, Ying, Talbot, Paul D, Glok, Mateusz K, Bernaś, Klaudia E, Thomson, Catherine M, Gould, Eoin R, Alphey, Magnus S, Liu, Huanting, Florence, Gordon J, Naismith, James H, da Silva, Rafael G

“…Adenosine 5′-triphosphate phosphoribosyltransferase (ATPPRT) catalyzes the first step in histidine biosynthesis, the condensation of ATP and…”
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Structural insights into the mechanism and inhibition of the β-hydroxydecanoyl-acyl carrier protein dehydratase from Pseudomonas aeruginosa by Moynié, Lucile, Leckie, Stuart M, McMahon, Stephen A, Duthie, Fraser G, Koehnke, Alessa, Taylor, James W, Alphey, Magnus S, Brenk, Ruth, Smith, Andrew D, Naismith, James H

“…Fatty acid biosynthesis is an essential component of metabolism in both eukaryotes and prokaryotes. The fatty acid biosynthetic pathway of Gram-negative…”
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Allosteric Competitive Inhibitors of the Glucose-1-phosphate Thymidylyltransferase (RmlA) from Pseudomonas aeruginosa by Alphey, Magnus S, Pirrie, Lisa, Torrie, Leah S, Boulkeroua, Wassila Abdelli, Gardiner, Mary, Sarkar, Aurijit, Maringer, Marko, Oehlmann, Wulf, Brenk, Ruth, Scherman, Michael S, McNeil, Michael, Rejzek, Martin, Field, Robert A, Singh, Mahavir, Gray, David, Westwood, Nicholas J, Naismith, James H

“…Glucose-1-phosphate thymidylyltransferase (RmlA) catalyzes the condensation of glucose-1-phosphate (G1P) with deoxy-thymidine triphosphate (dTTP) to yield…”
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High resolution crystal structures of Siglec-7. Insights into ligand specificity in the Siglec family by Alphey, Magnus S, Attrill, Helen, Crocker, Paul R, van Aalten, Daan M F

“…Sialic acid-binding immunoglobulin-like lectins (Siglecs) recognize sialylated glycoconjugates and play a role in cell-cell recognition. Siglec-7 is expressed…”
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Recombinant Human PPAR-β/δ Ligand-binding Domain is Locked in an Activated Conformation by Endogenous Fatty Acids by Fyffe, Stewart A., Alphey, Magnus S., Buetow, Lori, Smith, Terry K., Ferguson, Michael A.J., Sørensen, Morten D., Björkling, Fredrik, Hunter, William N.

“…High-resolution crystallographic structures of recombinant human peroxisome proliferator-activated receptor ligand-binding domain (isotype β/δ) reveal a fatty…”
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