Search Results - "Alpert, Andrew J"

Effect of salts on retention in hydrophilic interaction chromatography by Alpert, Andrew J.

“…•Hydration of counterions can strongly affect retention of charged solutes in HILIC.•Salting-out can occur at high salt concentration.•Electrostatic effects…”
Get full text

Electrostatic Repulsion Hydrophilic Interaction Chromatography for Isocratic Separation of Charged Solutes and Selective Isolation of Phosphopeptides by Alpert, Andrew J

“…If an ion-exchange column is eluted with a predominantly organic mobile phase, then solutes can be retained through hydrophilic interaction even if they have…”
Get full text

Anion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion–Hydrophilic Interaction Chromatography by Alpert, Andrew J, Hudecz, Otto, Mechtler, Karl

“…Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the…”
Get full text

Top-Down Proteomics of Large Proteins up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy by Cai, Wenxuan, Tucholski, Trisha, Chen, Bifan, Alpert, Andrew J, McIlwain, Sean, Kohmoto, Takushi, Jin, Song, Ge, Ying

“…Mass spectrometry (MS)-based top-down proteomics is a powerful method for the comprehensive analysis of proteoforms that arise from genetic variations and…”
Get full text

Online Hydrophobic Interaction Chromatography–Mass Spectrometry for the Analysis of Intact Monoclonal Antibodies by Chen, Bifan, Lin, Ziqing, Alpert, Andrew J, Fu, Cexiong, Zhang, Qunying, Pritts, Wayne A, Ge, Ying

“…Therapeutic monoclonal antibodies (mAbs) are an important class of drugs for a wide spectrum of human diseases. Liquid chromatography (LC) coupled to mass…”
Get full text

Structure and dynamics of endogenous cardiac troponin complex in human heart tissue captured by native nanoproteomics by Chapman, Emily A., Roberts, David S., Tiambeng, Timothy N., Andrews, Jãán, Wang, Man-Di, Reasoner, Emily A., Melby, Jake A., Li, Brad H., Kim, Donguk, Alpert, Andrew J., Jin, Song, Ge, Ying

“…Protein complexes are highly dynamic entities that display substantial diversity in their assembly, post-translational modifications, and non-covalent…”
Get full text

Peptide Orientation Affects Selectivity in Ion-Exchange Chromatography by Alpert, Andrew J, Petritis, Konstantinos, Kangas, Lars, Smith, Richard D, Mechtler, Karl, Mitulović, Goran, Mohammed, Shabaz, Heck, Albert J. R

“…Here we demonstrate that separation of proteolytic peptides, having the same net charge and one basic residue, is affected by their specific orientation toward…”
Get full text

Online Hydrophobic Interaction Chromatography–Mass Spectrometry for Top-Down Proteomics by Chen, Bifan, Peng, Ying, Valeja, Santosh G, Xiu, Lichen, Alpert, Andrew J, Ge, Ying

“…Recent progress in top-down proteomics has led to a demand for mass spectrometry (MS)-compatible chromatography techniques to separate intact proteins using…”
Get full text

Counterion Optimization Dramatically Improves Selectivity for Phosphopeptides and Glycopeptides in Electrostatic Repulsion-Hydrophilic Interaction Chromatography by Cui, Yusi, Tabang, Dylan Nicholas, Zhang, Zishan, Ma, Min, Alpert, Andrew J, Li, Lingjun

“…A well-hydrated counterion can selectively and dramatically increase retention of a charged analyte in hydrophilic interaction chromatography. The effect is…”
Get full text

Detection, Evaluation and Minimization of Nonenzymatic Deamidation in Proteomic Sample Preparation by Hao, Piliang, Ren, Yan, Alpert, Andrew J., Sze, Siu Kwan

“…Identification of deamidated sites in proteins is commonly used for assignment of N-glycosylation sites. It is also important for assessing the role of…”
Get full text

Top-Down Proteomics of Endogenous Membrane Proteins Enabled by Cloud Point Enrichment and Multidimensional Liquid Chromatography–Mass Spectrometry by Brown, Kyle A, Tucholski, Trisha, Alpert, Andrew J, Eken, Christian, Wesemann, Lucas, Kyrvasilis, Andreas, Jin, Song, Ge, Ying

“…Although top-down proteomics has emerged as a powerful strategy to characterize proteins in biological systems, the analysis of endogenous membrane proteins…”
Get full text

Protein Fractionation and Enrichment Prior to Proteomics Sample Preparation by Alpert, Andrew J

“…Proteins may be considered as polypeptides large enough to have a well-defined tertiary, or three-dimensional structure. In aqueous media, this structure is…”
Get more information

Effective Protein Separation by Coupling Hydrophobic Interaction and Reverse Phase Chromatography for Top-down Proteomics by Xiu, Lichen, Valeja, Santosh G, Alpert, Andrew J, Jin, Song, Ge, Ying

“…One of the challenges in proteomics is the proteome’s complexity, which necessitates the fractionation of proteins prior to the mass spectrometry (MS)…”
Get full text

Bullet points to evaluate the performance of the middle-down proteomics workflow for histone modification analysis by Coradin, Mariel, Mendoza, Mariel R., Sidoli, Simone, Alpert, Andrew J., Lu, Congcong, Garcia, Benjamin A.

“…•Histone post-translational modifications are investigated using WCX-HILIC.•PGC is utilized during sample desalting to reduce sample loss.•Evaluation of the…”
Get full text

HILIC at 21: Reflections and perspective by Alpert, Andrew J.

Get full text

Small-Scale Serial Size Exclusion Chromatography (s3SEC) for High Sensitivity Top-Down Proteomics of Large Proteoforms by Rogers, Holden T., Melby, Jake A., Ehlers, Lauren E., Fischer, Matthew S., Larson, Eli J., Gao, Zhan, Rossler, Kalina J., Wang, Daojing, Alpert, Andrew J., Ge, Ying

“…Top-down-mass spectrometry (MS)-based proteomics has emerged as a premier technology to examine proteins at the proteoform level, enabling characterization of…”
Get full text

Hydrophobic Interaction Chromatography for Bottom-Up Proteomics Analysis of Single Proteins and Protein Complexes by Rackiewicz, Michal, Große-Hovest, Ludger, Alpert, Andrew J, Zarei, Mostafa, Dengjel, Jörn

“…Hydrophobic interaction chromatography (HIC) is a robust standard analytical method to purify proteins while preserving their biological activity. It is widely…”
Get full text

Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds by Alpert, A J

“…When a hydrophilic chromatography column is eluted with a hydrophobic (mostly organic) mobile phase, retention increases with hydrophilicity of solutes. The…”
Get more information

Hydrophilic-interaction chromatography of complex carbohydrates by Alpert, A J, Shukla, M, Shukla, A K, Zieske, L R, Yuen, S W, Ferguson, M A, Mehlert, A, Pauly, M, Orlando, R

“…Complex carbohydrates can frequently be separated using hydrophilic-interaction chromatography (HILIC). The mechanism was investigated using small…”
Get more information

Ion-chromatography performance of poly(aspartic) acid bonded silica with various pore sizes at different ionic strengths and column temperatures by KEBETS, Pavel A, NESTERENKO, Ekaterina P, NESTERENKO, Pavel N, ALPERT, Andrew J

“…A significant effect of pore size on selec-tivity was observed for anion exchange on polyaspartic acid-coated silica (PolyCAT A). The retention order of…”
Get full text