Search Results - "Alperovich, Nina"

Single-cell measurement of plasmid copy number and promoter activity by Shao, Bin, Rammohan, Jayan, Anderson, Daniel A., Alperovich, Nina, Ross, David, Voigt, Christopher A.

“…Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA,…”
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Automation protocol for high-efficiency and high-quality genomic DNA extraction from Saccharomyces cerevisiae by Alperovich, Nina, Scott, Benjamin M, Ross, David

“…Although many protocols have been previously developed for genomic DNA (gDNA) extraction from S . cerevisiae , to take advantage of recent advances in…”
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Precision engineering of biological function with large-scale measurements and machine learning by Tack, Drew S, Tonner, Peter D, Pressman, Abe, Olson, Nathan D, Levy, Sasha F, Romantseva, Eugenia F, Alperovich, Nina, Vasilyeva, Olga, Ross, David

“…As synthetic biology expands and accelerates into real-world applications, methods for quantitatively and precisely engineering biological function become…”
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The genotype‐phenotype landscape of an allosteric protein by Tack, Drew S, Tonner, Peter D, Pressman, Abe, Olson, Nathan D, Levy, Sasha F, Romantseva, Eugenia F, Alperovich, Nina, Vasilyeva, Olga, Ross, David

“…Allostery is a fundamental biophysical mechanism that underlies cellular sensing, signaling, and metabolism. Yet a quantitative understanding of allosteric…”
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Genome Transplantation in Bacteria: Changing One Species to Another by Lartigue, Carole, Glass, John I, Alperovich, Nina, Pieper, Rembert, Parmar, Prashanth P, Hutchison, Clyde A. III, Smith, Hamilton O, Venter, J. Craig

“…As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole…”
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Essential Genes of a Minimal Bacterium by Glass, John I., Assad-Garcia, Nacyra, Alperovich, Nina, Yooseph, Shibu, Lewis, Matthew R., Maruf, Mahir, Hutchison, Clyde A., Smith, Hamilton O., Venter, J. Craig

“…Mycoplasma genitalium has the smallest genome of any organism that can be grown in pure culture. It has a minimal metabolism and little genomic redundancy…”
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Synthetic generation of influenza vaccine viruses for rapid response to pandemics by Dormitzer, Philip R, Suphaphiphat, Pirada, Gibson, Daniel G, Wentworth, David E, Stockwell, Timothy B, Algire, Mikkel A, Alperovich, Nina, Barro, Mario, Brown, David M, Craig, Stewart, Dattilo, Brian M, Denisova, Evgeniya A, De Souza, Ivna, Eickmann, Markus, Dugan, Vivien G, Ferrari, Annette, Gomila, Raul C, Han, Liqun, Judge, Casey, Mane, Sarthak, Matrosovich, Mikhail, Merryman, Chuck, Palladino, Giuseppe, Palmer, Gene A, Spencer, Terika, Strecker, Thomas, Trusheim, Heidi, Uhlendorff, Jennifer, Wen, Yingxia, Yee, Anthony C, Zaveri, Jayshree, Zhou, Bin, Becker, Stephan, Donabedian, Armen, Mason, Peter W, Glass, John I, Rappuoli, Rino, Venter, J Craig

“…During the 2009 H1N1 influenza pandemic, vaccines for the virus became available in large quantities only after human infections peaked. To accelerate vaccine…”
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Comparison of bias and resolvability in single-cell and single-transcript methods by Rammohan, Jayan, Lund, Steven P., Alperovich, Nina, Paralanov, Vanya, Strychalski, Elizabeth A., Ross, David

“…Single-cell and single-transcript measurement methods have elevated our ability to understand and engineer biological systems. However, defining and comparing…”
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Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination by Vashee, Sanjay, Stockwell, Timothy B, Alperovich, Nina, Denisova, Evgeniya A, Gibson, Daniel G, Cady, Kyle C, Miller, Kristofer, Kannan, Krishna, Malouli, Daniel, Crawford, Lindsey B, Voorhies, Alexander A, Bruening, Eric, Caposio, Patrizia, Früh, Klaus

“…Genetic engineering of cytomegalovirus (CMV) currently relies on generating a bacterial artificial chromosome (BAC) by introducing a bacterial origin of…”
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Erratum To: The genotype‐phenotype landscape of an allosteric protein by Tack, Drew S, Tonner, Peter D, Pressman, Abe, Olson, Nathan D, Levy, Sasha F, Romantseva, Eugenia F, Alperovich, Nina, Vasilyeva, Olga, Ross, David

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Comparison of Bias and Resolvability in Single-Cell and Single-Transcript Methods by Rammohan, Jayan, Lund, Steven P., Alperovich, Nina, Paralanov, Vanya, Strychalski, Elizabeth A., Ross, David

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Best Practices for DNA Template Preparation Toward Improved Reproducibility in Cell-Free Protein Production by Romantseva, Eugenia F, Tack, Drew S, Alperovich, Nina, Ross, David, Strychalski, Elizabeth A

“…Performance variability is a common challenge in cell-free protein production and hinders a wider adoption of these systems for both research and…”
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Genome-wide engineering of an infectious clone of herpes simplex virus type 1 using synthetic genomics assembly methods by Oldfield, Lauren M., Grzesik, Peter, Voorhies, Alexander A., Alperovich, Nina, MacMath, Derek, Najera, Claudia D., Chandra, Diya Sabrina, Prasad, Sanjana, Noskov, Vladimir N., Montague, Michael G., Friedman, Robert M., Desai, Prashant J., Vashee, Sanjay

“…Here, we present a transformational approach to genome engineering of herpes simplex virus type 1 (HSV-1), which has a large DNA genome, using synthetic…”
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The genotype‐phenotype landscape of an allosteric protein by Tack, Drew S, Tonner, Peter D, Pressman, Abe, Olson, Nathan D, Levy, Sasha F, Romantseva, Eugenia F, Alperovich, Nina, Vasilyeva, Olga, Ross, David

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Creating Bacterial Strains from Genomes That Have Been Cloned and Engineered in Yeast by Lartigue, Carole, Vashee, Sanjay, Algire, Mikkel A, Chuang, Ray-Yuan, Benders, Gwynedd A, Ma, Li, Noskov, Vladimir N, Denisova, Evgeniya A, Gibson, Daniel G, Assad-Garcia, Nacyra, Alperovich, Nina, Thomas, David W, Merryman, Chuck, Hutchison, Clyde A. III, Smith, Hamilton O, Venter, J. Craig, Glass, John I

“…We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. To produce a synthetic cell, the genome must be transferred…”
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Simultaneous non-contiguous deletions using large synthetic DNA and site-specific recombinases by Krishnakumar, Radha, Grose, Carissa, Haft, Daniel H, Zaveri, Jayshree, Alperovich, Nina, Gibson, Daniel G, Merryman, Chuck, Glass, John I

“…Toward achieving rapid and large scale genome modification directly in a target organism, we have developed a new genome engineering strategy that uses a…”
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Cloning whole bacterial genomes in yeast by Benders, Gwynedd A, Noskov, Vladimir N, Denisova, Evgeniya A, Lartigue, Carole, Gibson, Daniel G, Assad-Garcia, Nacyra, Chuang, Ray-Yuan, Carrera, William, Moodie, Monzia, Algire, Mikkel A, Phan, Quang, Alperovich, Nina, Vashee, Sanjay, Merryman, Chuck, Venter, J. Craig, Smith, Hamilton O, Glass, John I, Hutchison, Clyde A. III

“…Most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically…”
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Effects of DNA template preparation on variability in cell-free protein production by Romantseva, Eugenia, Alperovich, Nina, Ross, David, Lund, Steven P, Strychalski, Elizabeth A

“…Abstract DNA templates for protein production remain an unexplored source of variability in the performance of cell-free expression (CFE) systems. To…”
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Multicolor plate reader fluorescence calibration by Beal, Jacob, Telmer, Cheryl A, Vignoni, Alejandro, Boada, Yadira, Baldwin, Geoff S, Hallett, Liam, Lee, Taeyang, Selvarajah, Vinoo, Billerbeck, Sonja, Brown, Bradley, Cai, Guo-nan, Cai, Liang, Eisenstein, Edward, Kiga, Daisuke, Ross, David, Alperovich, Nina, Sprent, Noah, Thompson, Jaclyn, Young, Eric M, Endy, Drew, Haddock-Angelli, Traci

“…Abstract Plate readers are commonly used to measure cell growth and fluorescence, yet the utility and reproducibility of plate reader data is limited by the…”
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