CRISPR‐Cas enzymes: The toolkit revolutionizing diagnostics

The programmable nature of sequence‐specific targeting by CRISPR‐Cas nucleases has revolutionized a wide range of genomic applications and is now emerging as a method for nucleic acid detection. We explore how the diversity of CRISPR systems and their fundamental mechanisms have given rise to a wave...

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Bibliographic Details
Published in:	Biotechnology journal Vol. 17; no. 7; pp. e2100304 - n/a
Main Authors:	Verosloff, Matthew S., Shapiro, Sarah J., Hawkins, Elizabeth M., Alpay, Emel, Verma, Deepika, Stanfield, Emma G., Kreindler, Lior, Jain, Sonal, McKay, Bridget, Hubbell, Shae A., Hendriks, Carley G., Blizard, Benjamin A., Broughton, James P., Chen, Janice S.
Format:	Journal Article
Language:	English
Published:	01-07-2022
Subjects:	biochemistry bioengineering CRISPR diagnostics CRISPR‐Cas proteins nucleic acid detection
Online Access:	Get full text
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Summary:	The programmable nature of sequence‐specific targeting by CRISPR‐Cas nucleases has revolutionized a wide range of genomic applications and is now emerging as a method for nucleic acid detection. We explore how the diversity of CRISPR systems and their fundamental mechanisms have given rise to a wave of new methods for target recognition and readout. These cross‐disciplinary advances found at the intersection of CRISPR biology and engineering have led to the ability to rapidly generate solutions for emerging global challenges like the COVID‐19 pandemic. We further discuss the advances and potential for CRISPR‐based detection to have an impact across a continuum of diagnostic applications. Graphical and Lay Summary The inherent programmability of CRISPR‐Cas nucleases has enabled new methods for molecular diagnostics. Powered by the intersection of CRISPR biology and engineering, CRISPR‐based diagnostics offer timely, accurate and actionable solutions across the continuum of testing applications.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1860-6768 1860-7314
DOI:	10.1002/biot.202100304