Cenococcum geophilum impedes cadmium toxicity in Pinus massoniana by modulating nitrogen metabolism

Cenococcum geophilum impedes cadmium toxicity in Pinus massoniana by modulating nitrogen metabolism

Nitrogen (N) is of great significance to the absorption, distribution and detoxification of cadmium (Cd). Ectomycorrhizal fungi (EMF) are able to affect the key processes of plant N uptake to resist Cd stress, while the mechanism is still unclear. Therefore, we explored potential strategies of Cenoc...

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Bibliographic Details
Published in:The Science of the total environment Vol. 946; p. 174296
Main Authors: Zhang, Panpan, Zhang, Yuhu, Pang, Wenbo, Alonazi, Madeha A., Alwathnani, Hend, Rensing, Christopher, Xie, Rongzhang, Zhang, Taoxiang
Format: Journal Article
Language:English
Published: Netherlands Elsevier B.V 10-10-2024
Subjects:
Cadmium stress
Ectomycorrhizal fungi
Nitrogen assimilation
Nitrogen transport
Nitrogen transport
Cadmium stress
Nitrogen assimilation
Ectomycorrhizal fungi
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Summary:Nitrogen (N) is of great significance to the absorption, distribution and detoxification of cadmium (Cd). Ectomycorrhizal fungi (EMF) are able to affect the key processes of plant N uptake to resist Cd stress, while the mechanism is still unclear. Therefore, we explored potential strategies of Cenococcum geophilum (C. geophilum) symbiosis to alleviate Cd stress in Pinus massoniana (P. massoniana) from the perspective of plant N metabolism and soil N transformation. The results showed that inoculation of C. geophilum significantly increased the activities of NR, NiR and GS in the shoots and roots of P. massoniana, thereby promoting the assimilation of NO3− and NH4+ into amino acids. Moreover, C. geophilum promoted soil urease and protease activities, but decreased soil NH4+ content, indicating that C. geophilum might increase plant uptake of soil inorganic N. qRT-PCR results showed that C3 symbiosis significantly up-regulated the expression of genes encoding functions involved in NH4+ uptake (AMT3;1), NO3− uptake (NRT2.1, NRT2.4, NRT2.9), as well as Cd resistance (ABCC1 and ABCC2), meanwhile down-regulated the expression of NRT7.3, Cd transporter genes (HMA2 and NRAMP3) in the roots of P. massoniana seedlings. These results demonstrated that C. geophilum was able to alleviate Cd stress by increasing the absorption and assimilation of inorganic N in plants and inhibiting the transport of Cd from roots to shoots, which provided new insights into how EMF improved host resistance to abiotic stress. [Display omitted] •C. geophilum promoted N metabolism to help P. massoniana resist Cd stress.•C. geophilum inoculation promoted the expression of plant N transport genes.•C. geophilum increased the activity of soil N conversion enzymes to promote the availability of nitrogen in soil.
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ISSN:0048-9697
1879-1026
1879-1026
DOI:10.1016/j.scitotenv.2024.174296