Search Results - "Almers, W"

A real-time view of life within 100 nm of the plasma membrane by Steyer, Juergen A, Almers, Wolf

“…The plasma membrane is a two-dimensional compartment that relays most biological signals sent or received by a cell. Signalling involves membrane receptors and…”
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Transport, capture and exocytosis of single synaptic vesicles at active zones by Almers, W, Zenisek, D, Steyer, J. A

“…To sustain high rates of transmitter release, synaptic terminals must rapidly re-supply vesicles to release sites and prime them for exocytosis. Here we…”
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Transport, docking and exocytosis of single secretory granules in live chromaffin cells by Almers, W, Steyer, J. A, Horstmann, H

“…Neurons maintain a limited pool of synaptic vesicles which are docked at active zones and are awaiting exocytosis. By contrast, endocrine cells releasing…”
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Tracking Single Secretory Granules in Live Chromaffin Cells by Evanescent-Field Fluorescence Microscopy by Steyer, J.A., Almers, W.

“…We have observed secretory granules beneath the plasma membrane of chromaffin cells. Using evanescent-field excitation by epiillumination, we have illuminated…”
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The exocytotic event in chromaffin cells revealed by patch amperometry by Lindau, M, Albillos, A, Dernick, G, Horstmann, H, Almers, W, de Toledo, G. Alvarez

“…In mast cells and granulocytes, exocytosis starts with the formation of a fusion pore,,. It has been suggested that neurotransmitters may be released through…”
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Exocytosis by ALMERS, W

“…Previous articles have discussed why artificial lipid bilayers are so resistant to fusion, how this resistance may be overcome, and what the findings with pure…”
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Docked granules, the exocytic burst, and the need for ATP hydrolysis in endocrine cells by Parsons, T.D., Coorssen, J.R., Horstmann, H., Almers, W.

“…Ca 2+-triggered exocytosis was studied in single rat melanotrophs and bovine chromaffin cells by capacitance measurements. Sustained exocytosis required MgATP,…”
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Fusion needs more than SNAREs by Almers, Wolfhard

“…Force biological membranes close enough together and they will fuse. SNARE proteins are well suited to force proximity, but biochemical studies of yeast show…”
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Local Ca2+ Release from Internal Stores Controls Exocytosis in Pituitary Gonadotrophs by Tse, F.W., Tse, A., Hille, B., Horstmann, H., Almers, W.

“…Exocytosis and the cell-averaged cytosolic [Ca2+], [Ca2+]i, were tracked in single gonadotrophs. Cells released 100 granules/s at 1 μM = [Ca2+]i when…”
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Ca2+ triggers massive exocytosis in Chinese hamster ovary cells by Coorssen, J. R., Schmitt, H., Almers, W.

“…We have tracked the cell surface area of CHO cells by measuring the membrane capacitance, Cm. An increase in cytosolic [Ca2+], [Ca2+]i, increased the cell…”
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A triggered mechanism retrieves membrane in seconds after Ca(2+)-stimulated exocytosis in single pituitary cells by Thomas, P, Lee, A K, Wong, J G, Almers, W

“…In neuroendocrine cells, cytosolic Ca2+ triggers exocytosis in tens of milliseconds, yet known pathways of endocytic membrane retrieval take minutes. To test…”
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Membrane Flux through the Pore Formed by a Fusogenic Viral Envelope Protein during Cell Fusion by Tse, F. W., Iwata, A., Almers, W.

“…We have investigated the mechanism of cell fusion mediated by HA, the fusogenic hemagglutinin of the Influenza viral envelope. Single erythrocytes (RBCs) were…”
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A Triggered Mechanism Retrieves Membrane in Seconds after Ca2+-Stimulated Exocytosis in Single Pituitary Cells by Thomas, P., Lee, A. K., Wong, J. G., Almers, W.

“…In neuroendocrine cells, cytosolic Ca2+ triggers exocytosis in tens of milliseconds, yet known pathways of endocytic membrane retrieval take minutes. To test…”
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A low affinity Ca2+ receptor controls the final steps in peptide secretion from pituitary melanotrophs by Thomas, P, Wong, J G, Lee, A K, Almers, W

“…Using flash photolysis of caged Ca2+ and the membrane capacitance to monitor exocytosis, we have studied the response of single melanotrophs to a step rise in…”
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Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells by Henkel, A.W., Meiri, H., Horstmann, H., Lindau, M., Almers, W.

“…Constitutive exo‐ and endocytic events are expected to increase and diminish the cell surface area in small spontaneous steps. Indeed, cell‐attached…”
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The Ca signal from fura-2 loaded mast cells depends strongly on the method of dye-loading by Almers, W., Neher, E.

“…The Ca concentration ([Ca 2+] i) in single rat peritoneal mast cells was measured by means of the new fluorescent Ca-indicator dye fura-2. Dye-loaded cells…”
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The First Milliseconds of the Pore Formed by a Fusogenic Viral Envelope Protein During Membrane Fusion by SPRUCE, A. E, IWATA, A, ALMERS, W

“…Fibroblasts expressing the influenza virus hemagglutinin on their plasma membrane were patch clamped while they fused to erythrocytes. An increase in the…”
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Currents through the fusion pore that forms during exocytosis of a secretory vesicle by Breckenridge, L. J, Almers, W

“…Exocytosis, or the fusion of cytoplasmic vesicles with the cell membrane, occurs in nearly all eukaryotic cells, but its mechanism is not understood…”
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Non-selective conductance in calcium channels of frog muscle: calcium selectivity in a single-file pore by Almers, W, McCleskey, E W

“…Voltage-clamp studies were carried out to compare currents through Ca2+ channels (ICa) with Na+ currents (Ins) through a non-selective cation conductance…”
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Millisecond studies of secretion in single rat pituitary cells stimulated by flash photolysis of caged Ca2 by Thomas, P., Wong, J.G., Almers, W.

“…To study the final steps in the secretory pathway of rat pituitary melanotrophs, we have monitored changes in cell surface area due to exocytosis after flash…”
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