Development, Optimization, and Validation of an Ultrasound-Assisted Liquid–Liquid Microextraction (UALLME) for Selenomethionine Analyses in Cashew Nut (Anacardium occidentale) by Ultra-Performance Liquid Chromatography Coupled to Electrospray Ionization/Single Quadrupole Mass Spectrometer (UPLC-ESI/QDa)

Development, Optimization, and Validation of an Ultrasound-Assisted Liquid–Liquid Microextraction (UALLME) for Selenomethionine Analyses in Cashew Nut (Anacardium occidentale) by Ultra-Performance Liquid Chromatography Coupled to Electrospray Ionization/Single Quadrupole Mass Spectrometer (UPLC-ESI/QDa)

A method based on liquid–liquid microextraction assisted by ultrasound energy (UALLME) and UPLC-ESI/QDa detection was developed for SeMet analysis in cashew nut samples. The UALLME conditions were optimized through an experimental design of three factors in two levels with central point, which was o...

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Bibliographic Details
Published in:Food analytical methods Vol. 15; no. 11; pp. 3196 - 3208
Main Authors: Alcântara, Daniel B., Riceli, Paulo, Almeida, Ana Debora S., Luz, Lícia R., Nascimento, Hélio O., Fernandes, Tatiana S. M., Dionísio, Ana Paula, Castro, Ana Cecília R., Nascimento, Ronaldo F., Lopes, Gisele S., Zocolo, Guilherme J.
Format: Journal Article
Language:English
Published: New York Springer US 01-11-2022
Springer Nature B.V
Subjects:
Anacardiaceae
Analytical Chemistry
Calibration
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Design factors
Design of experiments
Design optimization
Experimental design
Extreme values
Food Science
Genistein
Ionization
Linearity
Liquid chromatography
Mathematical analysis
Microbiology
Nuts
pH effects
Quadrupoles
Selectivity
Selenium
Selenomethionine
Solvents
Strong interactions (field theory)
Ultrasonic imaging
Ultrasound
Yeasts
SeMet
UPLC-ESI
Cashew Nut
UALLME
QDa
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Summary:A method based on liquid–liquid microextraction assisted by ultrasound energy (UALLME) and UPLC-ESI/QDa detection was developed for SeMet analysis in cashew nut samples. The UALLME conditions were optimized through an experimental design of three factors in two levels with central point, which was observed the highest yield of SeMet extraction from selenized yeast CRM when working with extracting solvent in extreme values of pH; also, occurrence of strong interaction between the variables “sample mass and extracting solvent pH” in the analyte response was detected. For the instrumental analysis, genistein showed as a suitable SeMet internal standard when was compared its efficiency in correcting the analyte signal with the SeMet similar compound (Se-methylselenocystin—SeMeSeC). As coextractives from the sample matrix influenced the analysis of the target compound by an overestimation of the analytical signal, the matrix-matched calibration strategy must be used for quantification. The method was validated and presented appropriate sensitivity, accuracy, instrumental, intra-assay and intra-day precisions, linearity, and linear range. The selectivity was guaranteed performing quantifications in the selected ion recording (SIR) acquisition mode. Analyses in commercial samples from three different brands demonstrated a probable absence of the analyte; however, the authors highlight the need to expand the research about the presence of SeMet in cashew nuts due to the importance of this nut to the economy of the state of Ceará and the benefits of SeMet to human health.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-022-02355-7