CHASE-Containing Histidine Kinase Receptors in Apple Tree: From a Common Receptor Structure to Divergent Cytokinin Binding Properties and Specific Functions

Cytokinin signaling is a key regulatory pathway of many aspects in plant development and environmental stresses. Herein, we initiated the identification and functional characterization of the five CHASE-containing histidine kinases (CHK) in the economically important species. These cytokinin recepto...

Full description

Saved in:

Bibliographic Details
Published in:	Frontiers in plant science Vol. 8; p. 1614
Main Authors:	Daudu, Dimitri, Allion, Elsa, Liesecke, Franziska, Papon, Nicolas, Courdavault, Vincent, Dugé de Bernonville, Thomas, Mélin, Céline, Oudin, Audrey, Clastre, Marc, Lanoue, Arnaud, Courtois, Martine, Pichon, Olivier, Giron, David, Carpin, Sabine, Giglioli-Guivarc'h, Nathalie, Crèche, Joël, Besseau, Sébastien, Glévarec, Gaëlle
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers 20-09-2017 Frontiers Media S.A
Subjects:	CHASE-containing histidine kinase cytokinin Life Sciences Malus domestica Plant Science protein–protein interaction RNAseq data Vegetal Biology yeast complementation assay protein–protein interaction Malus domestica RNAseq data cytokinin yeast complementation assay CHASE-containing histidine kinase pommier histidine kinase cytokinine récepteur de cytokine expression des gènes RNA-seq agent pathogène pathogen apple tree
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Cytokinin signaling is a key regulatory pathway of many aspects in plant development and environmental stresses. Herein, we initiated the identification and functional characterization of the five CHASE-containing histidine kinases (CHK) in the economically important species. These cytokinin receptors named MdCHK2, MdCHK3a/MdCHK3b, and MdCHK4a/MdCHK4b by homology with AHK clearly displayed three distinct profiles. The three groups exhibited architectural variations, especially in the N-terminal part including the cytokinin sensing domain. Using a yeast complementation assay, we showed that MdCHK2 perceives a broad spectrum of cytokinins with a substantial sensitivity whereas both MdCHK4 homologs exhibit a narrow spectrum. Both MdCHK3 homologs perceived some cytokinins but surprisingly they exhibited a basal constitutive activity. Interaction studies revealed that MdCHK2, MdCHK4a, and MdCHK4b homodimerized whereas MdCHK3a and MdCHK3b did not. Finally, qPCR analysis and bioinformatics approach pointed out contrasted expression patterns among the three MdCHK groups as well as distinct sets of co-expressed genes. Our study characterized for the first time the five cytokinin receptors in apple tree and provided a framework for their further functional studies.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Ján A. Miernyk, Agricultural Research Service (USDA), United States This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science Reviewed by: Jan Hejatko, Masaryk University, Czechia; Dong Xu, University of Missouri, United States
ISSN:	1664-462X 1664-462X
DOI:	10.3389/fpls.2017.01614