Comparison of methods to recover amaranth weed seeds from manure

Comparison of methods to recover amaranth weed seeds from manure

One pathway by which Palmer amaranth (Amaranthus palmeri S. Watson) invades new areas is through importation of contaminated livestock feed, which then contaminates land‐applied manure. If contaminated feed is suspected, detection tools are needed to test manure, but traditional methods are time con...

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Bibliographic Details
Published in:Agricultural & environmental letters Vol. 7; no. 1
Main Authors: Wilson, Melissa L., Brusa, Anthony, Christensen, Hatley, Strack, Samuel, Alto, Eddie, Allen, Luis F., Cortus, Scott D., Modderman, Chryseis, Becker, Roger L.
Format: Journal Article
Language:English
Published: Hoboken John Wiley & Sons, Inc 2022
Wiley
Subjects:
Aluminum
Amaranth
Amaranthus palmeri
Blending
Cattle manure
Contamination
Deoxyribonucleic acid
Dispersion
DNA
Drinking water
Factory farming
Feeds
Genetic screening
Importation
Land pollution
Livestock
Livestock feed
Livestock feeds
Manures
Methods
Polymerase chain reaction
Recovery
Seeds
United States > US
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Summary:One pathway by which Palmer amaranth (Amaranthus palmeri S. Watson) invades new areas is through importation of contaminated livestock feed, which then contaminates land‐applied manure. If contaminated feed is suspected, detection tools are needed to test manure, but traditional methods are time consuming and often inconclusive. Although new genetic seed testing is making detection easier, methods to separate seed from contaminated manure are needed. Six methods were compared for their ability to recover 100 Palmer amaranth seeds added to bedded or nonbedded cattle manure: dry sieving, rinse sieving, manure saturation sieving without blending and with blending, and dispersion sieving without blending and with blending. Seed recovery was highest (>90%) with the rinse sieving method regardless of manure type. The dispersion methods are not recommended as they recovered <24.7% of seeds. Following each method, genetic testing successfully identified Palmer amaranth presence, indicating no interference of recovery method with DNA extraction. Core Ideas  Six methods to recover amaranth seeds from bedded and nonbedded manure were compared.  Rinsing manure through sieves was the most reliable method, recovering >90% of seeds.  Genetic testing positively identified Palmer amaranth recovered from manure.  With these testing methods farmers can more rapidly take precautions to reduce risk of spread.
Bibliography:Assigned to Associate Editor David Jordan.
ISSN:2471-9625
2471-9625
DOI:10.1002/ael2.20065