Search Results - "Allbritton, Nancy L."

Creation of a spatially complex mucus bilayer on an in vitro colon model by Villegas-Novoa, Cecilia, Wang, Yuli, Sims, Christopher E., Allbritton, Nancy L.

“…The colonic epithelium is comprised of three-dimensional crypts (3D) lined with mucus secreted by a heterogeneous population of goblet cells. In this study, we…”
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A Microphysiological System with an Anaerobic Air‐Liquid Interface and Functional Mucus Layer for Coculture of Intestinal Bacteria and Primary Human Colonic Epithelium by Kim, Raehyun, Allbritton, Nancy L.

“…Coculture of intestinal bacteria with primary human intestinal epithelium provides a valuable tool for investigating host‐colon bacterial interactions and for…”
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"Fix and assay": separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative by Proctor, Angela, Allbritton, Nancy L

“…Chemical cytometry using capillary electrophoresis (CE) is a powerful tool for measuring single-cell enzyme activity. However, these measurements are often…”
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A technology of a different sort: microraft arrays by Cortés-Llanos, Belén, Wang, Yuli, Sims, Christopher E, Allbritton, Nancy L

“…A common procedure performed throughout biomedical research is the selection and isolation of biological entities such as organelles, cells and organoids from…”
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LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching by Fadero, Tanner C, Gerbich, Therese M, Rana, Kishan, Suzuki, Aussie, DiSalvo, Matthew, Schaefer, Kristina N, Heppert, Jennifer K, Boothby, Thomas C, Goldstein, Bob, Peifer, Mark, Allbritton, Nancy L, Gladfelter, Amy S, Maddox, Amy S, Maddox, Paul S

“…Fluorescence microscopy is a powerful approach for studying subcellular dynamics at high spatiotemporal resolution; however, conventional fluorescence…”
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A high-throughput platform for stem cell niche co-cultures and downstream gene expression analysis by Gracz, Adam D., Williamson, Ian A., Roche, Kyle C., Johnston, Michael J., Wang, Fengchao, Wang, Yuli, Attayek, Peter J., Balowski, Joseph, Liu, Xiao Fu, Laurenza, Ryan J., Gaynor, Liam T., Sims, Christopher E., Galanko, Joseph A., Li, Linheng, Allbritton, Nancy L., Magness, Scott T.

“…Stem cells reside in ‘niches’, where support cells provide critical signalling for tissue renewal. Culture methods mimic niche conditions and support the…”
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A High-Throughput Organoid Microinjection Platform to Study Gastrointestinal Microbiota and Luminal Physiology by Williamson, Ian A, Arnold, Jason W, Samsa, Leigh Ann, Gaynor, Liam, DiSalvo, Matthew, Cocchiaro, Jordan L, Carroll, Ian, Azcarate-Peril, M. Andrea, Rawls, John F, Allbritton, Nancy L, Magness, Scott T

“…Background & Aims The human gut microbiota is becoming increasingly recognized as a key factor in homeostasis and disease. The lack of physiologically relevant…”
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Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer by Wang, Yuli, Kim, Raehyun, Gunasekara, Dulan B, Reed, Mark I, DiSalvo, Matthew, Nguyen, Daniel L, Bultman, Scott J, Sims, Christopher E, Magness, Scott T, Allbritton, Nancy L

“…Background & Aims The successful culture of intestinal organoids has greatly enhanced our understanding of intestinal stem cell physiology and enabled the…”
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Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells by Wang, Yuli, DiSalvo, Matthew, Gunasekara, Dulan B, Dutton, Johanna, Proctor, Angela, Lebhar, Michael S, Williamson, Ian A, Speer, Jennifer, Howard, Riley L, Smiddy, Nicole M, Bultman, Scott J, Sims, Christopher E, Magness, Scott T, Allbritton, Nancy L

“…Background & Aims Three-dimensional organoid culture has fundamentally changed the in vitro study of intestinal biology enabling novel assays; however, its use…”
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Chemical fixation to arrest phospholipid signaling for chemical cytometry by Proctor, Angela, Sims, Christopher E., Allbritton, Nancy L.

“…•High quality separation of phosphatidylinositol isomers was developed.•Exogenously added fluorescent phospholipid is retained in cells after…”
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In vitro generation of colonic epithelium from primary cells guided by microstructures by Wang, Yuli, Ahmad, Asad A, Sims, Christopher E, Magness, Scott T, Allbritton, Nancy L

“…The proliferative compartment of the colonic epithelium in vivo is located in the basal crypt where colonic stem cells and transit-amplifying cells reside and…”
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IL-22 promotes mucin-type O-glycosylation and MATH1+ cell-mediated amelioration of intestinal inflammation by Singh, Ankita, Beaupre, Michael, Villegas-Novoa, Cecilia, Shiomitsu, Kiyoshi, Gaudino, Stephen J., Tawch, Suzanne, Damle, Ruhee, Kempen, Cody, Choudhury, Biswa, McAleer, Jeremy P., Sheridan, Brian S., Denoya, Paula, Blumberg, Richard S., Hearing, Patrick, Allbritton, Nancy L., Kumar, Pawan

“…The interleukin (IL)-22 cytokine can be protective or inflammatory in the intestine. It is unclear if IL-22 receptor (IL-22Ra1)-mediated protection involves a…”
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IL-22 promotes mucin-type O-glycosylation and MATH1+ cell-mediated amelioration of intestinal inflammation by Singh, Ankita, Beaupre, Michael, Villegas-Novoa, Cecilia, Shiomitsu, Kiyoshi, Gaudino, Stephen J., Tawch, Suzanne, Damle, Ruhee, Kempen, Cody, Choudhury, Biswa, McAleer, Jeremy P., Sheridan, Brian S., Denoya, Paula, Blumberg, Richard S., Hearing, Patrick, Allbritton, Nancy L., Kumar, Pawan

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An Integrated Chemical Cytometry Method: Shining a Light on Akt Activity in Single Cells by Mainz, Emilie R., Wang, Qunzhao, Lawrence, David S., Allbritton, Nancy L.

“…Tools to evaluate oncogenic kinase activity in small clinical samples have the power to guide precision medicine in oncology. Existing platforms have…”
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Benchtop micromolding of polystyrene by soft lithography by Wang, Yuli, Balowski, Joseph, Phillips, Colleen, Phillips, Ryan, Sims, Christopher E, Allbritton, Nancy L

“…Polystyrene (PS), a standard material for cell culture consumable labware, was molded into microstructures with high fidelity of replication by an elastomeric…”
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Bioengineered Systems and Designer Matrices That Recapitulate the Intestinal Stem Cell Niche by Wang, Yuli, Kim, Raehyun, Hinman, Samuel S, Zwarycz, Bailey, Magness, Scott T, Allbritton, Nancy L

“…The relationship between intestinal stem cells (ISCs) and the surrounding niche environment is complex and dynamic. Key factors localized at the base of the…”
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Evaluation of human primary intestinal monolayers for drug metabolizing capabilities by Speer, Jennifer E, Wang, Yuli, Fallon, John K, Smith, Philip C, Allbritton, Nancy L

“…The intestinal epithelium is a major site of drug metabolism in the human body, possessing enterocytes that house brush border enzymes and phase I and II drug…”
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Pronase E‑Based Generation of Fluorescent Peptide Fragments: Tracking Intracellular Peptide Fate in Single Cells by Mainz, Emilie R, Dobes, Nicholas C, Allbritton, Nancy L

“…The ability to track intracellular peptide proteolysis at the single cell level is of growing interest, particularly as short peptide sequences continue to…”
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Automated microarray platform for single-cell sorting and collection of lymphocytes following HIV reactivation by Cortés-Llanos, Belén, Jain, Vaibhav, Cooper-Volkheimer, Alicia, Browne, Edward P, Murdoch, David M, Allbritton, Nancy L

“…A promising strategy to cure HIV-infected individuals is to use latency reversing agents (LRAs) to reactivate latent viruses, followed by host clearance of…”
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In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment by Attayek, Peter J, Ahmad, Asad A, Wang, Yuli, Williamson, Ian, Sims, Christopher E, Magness, Scott T, Allbritton, Nancy L

“…The polarity of proliferative and differentiated cellular compartments of colonic crypts is believed to be specified by gradients of key mitogens and…”
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