Search Results - "Allbritton, Nancy"

Analytical Techniques for Single-Cell Biochemical Assays of Lipids by Yao, Ming, Vaithiyanathan, Manibarathi, Allbritton, Nancy L

“…Lipids are essential cellular components forming membranes, serving as energy reserves, and acting as chemical messengers. Dysfunction in lipid metabolism and…”
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In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels by Hinman, Samuel S., Wang, Yuli, Kim, Raehyun, Allbritton, Nancy L.

“…The large intestine, with its array of crypts lining the epithelium and diverse luminal contents, regulates homeostasis throughout the body. In vitro crypts…”
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Primary Cell-Derived Intestinal Models: Recapitulating Physiology by Dutton, Johanna S., Hinman, Samuel S., Kim, Raehyun, Wang, Yuli, Allbritton, Nancy L.

“…The development of physiologically relevant intestinal models fueled by breakthroughs in primary cell-culture methods has enabled successful recapitulation of…”
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Human 2D Crypt Model for Assaying Intestinal Stem Cell Proliferation and Differentiation by Wang, Yuli, Sims, Christopher E., Allbritton, Nancy L.

“…Intestine is a common site of adverse drug effects in clinical trials; thus, improved in vitro models for preclinical screening of pharmaceutical compounds are…”
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A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium by Wang, Yuli, Gunasekara, Dulan B, Reed, Mark I, DiSalvo, Matthew, Bultman, Scott J, Sims, Christopher E, Magness, Scott T, Allbritton, Nancy L

“…Abstract The human small intestinal epithelium possesses a distinct crypt-villus architecture and tissue polarity in which proliferative cells reside inside…”
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Photopatterned Membranes and Chemical Gradients Enable Scalable Phenotypic Organization of Primary Human Colon Epithelial Models by Hinman, Samuel S, Wang, Yuli, Allbritton, Nancy L

“…Biochemical gradients across the intestinal epithelium play a major role in governing intestinal stem cell compartmentalization, differentiation dynamics, and…”
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Creation of a spatially complex mucus bilayer on an in vitro colon model by Villegas-Novoa, Cecilia, Wang, Yuli, Sims, Christopher E., Allbritton, Nancy L.

“…The colonic epithelium is comprised of three-dimensional crypts (3D) lined with mucus secreted by a heterogeneous population of goblet cells. In this study, we…”
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Monolithic Silica Microbands Enable Thin-Layer Chromatography Analysis of Single Cells by Wang, Yuli, Yao, Ming, Sims, Christopher E., Allbritton, Nancy L.

“…A picoliter thin-layer chromatography (pTLC) platform was developed for analyzing extremely miniature specimens, such as assay of the contents of a single cell…”
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Enterochromaffin Cell-Enriched Monolayer Platform for Assaying Serotonin Release from Human Primary Intestinal Cells by Wang, Yuli, Sims, Christopher E, Allbritton, Nancy L

“…Enteroendocrine (EE) cells within the intestinal epithelium produce a range of hormones that have key roles in modulating satiety and feeding behavior in…”
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Silicon Photomultipliers as a Low-Cost Fluorescence Detector for Capillary Electrophoresis by Petersen, Brae V, Gallion, Luke, Allbritton, Nancy L

“…Capillary electrophoresis (CE) is a highly efficient separation method capable of handling small sample volumes (∼pL) and low (∼yoctomole) detection limits…”
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A Microphysiological System with an Anaerobic Air‐Liquid Interface and Functional Mucus Layer for Coculture of Intestinal Bacteria and Primary Human Colonic Epithelium by Kim, Raehyun, Allbritton, Nancy L.

“…Coculture of intestinal bacteria with primary human intestinal epithelium provides a valuable tool for investigating host‐colon bacterial interactions and for…”
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Image-Based Live Cell Sorting by LaBelle, Cody A., Massaro, Angelo, Cortés-Llanos, Belén, Sims, Christopher E., Allbritton, Nancy L.

“…Technologies capable of cell separation based on cell images provide powerful tools enabling cell selection criteria that rely on spatially or temporally…”
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"Fix and assay": separating in-cellulo sphingolipid reactions from analytical assay in time and space using an aldehyde-based fixative by Proctor, Angela, Allbritton, Nancy L

“…Chemical cytometry using capillary electrophoresis (CE) is a powerful tool for measuring single-cell enzyme activity. However, these measurements are often…”
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Development of a Primary Human Intestinal Epithelium Enriched in L‑Cells for Assay of GLP‑1 Secretion by Villegas-Novoa, Cecilia, Wang, Yuli, Sims, Christopher E., Allbritton, Nancy L.

“…Type 2 diabetes mellitus is a chronic disease associated with obesity and dysregulated human feeding behavior. The hormone glucagon-like peptide 1 (GLP-1), a…”
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Mucus-coated, magnetically-propelled fecal surrogate to mimic fecal shear forces on colonic epithelium by Wang, Alan S., Villegas-Novoa, Cecilia, Wang, Yuli, Sims, Christopher E., Allbritton, Nancy L.

“…The relationship between the mechanical forces associated with bowel movement and colonic mucosal physiology is understudied. This is partly due to the limited…”
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Integrative microphysiological tissue systems of cancer metastasis to the liver by Clark, Amanda M, Allbritton, Nancy L, Wells, Alan

“…The liver is the most commonly involved organ in metastases from a wide variety of solid tumors. The use of biologically and cellularly complex liver tissue…”
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Micro Total Analysis Systems for Cell Biology and Biochemical Assays by Kovarik, Michelle L, Gach, Philip C, Ornoff, Douglas M, Wang, Yuli, Balowski, Joseph, Farrag, Lila, Allbritton, Nancy L

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Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors by Wheeler, Emily C., Vu, Anthony Q., Einstein, Jaclyn M., DiSalvo, Matthew, Ahmed, Noorsher, Van Nostrand, Eric L., Shishkin, Alexander A., Jin, Wenhao, Allbritton, Nancy L., Yeo, Gene W.

“…Genetic screens using pooled CRISPR-based approaches are scalable and inexpensive, but restricted to standard readouts, including survival, proliferation and…”
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Analysis of single mammalian cells on-chip by Sims, Christopher E, Allbritton, Nancy L

“…A goal of modern biology is to understand the molecular mechanisms underlying cellular function. The ability to manipulate and analyze single cells is crucial…”
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A technology of a different sort: microraft arrays by Cortés-Llanos, Belén, Wang, Yuli, Sims, Christopher E, Allbritton, Nancy L

“…A common procedure performed throughout biomedical research is the selection and isolation of biological entities such as organelles, cells and organoids from…”
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