Molecular analysis of commercial date palm cultivars in Lybia using ISSR and SRAP PCR-based markers
Little is known about the molecular structure of the date palm (Phoenix dactylifera L.) despite its importance as invaluable drought tolerant crop. Intervarietal variation and cultivar identification are crucial for breeding and gene bank conservation of this plant worldwide. In this work, two PCR b...
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| Published in: | Genetika (Beograd) Vol. 48; no. 1; pp. 307 - 322 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
2016
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| Online Access: | Get full text |
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| Summary: | Little is known about the molecular structure of the date palm (Phoenix
dactylifera L.) despite its importance as invaluable drought tolerant crop.
Intervarietal variation and cultivar identification are crucial for breeding
and gene bank conservation of this plant worldwide. In this work, two PCR
based marker systems (ISSR and SRAP) were applied on top quality eight
commercial cultivars in Libya (Umfetity, Bekrary, Alhamraya, Sufeer Genab,
Alsaeedy Show, Farag Barameel, Majhool Alheelo and Alkhadraya). DNA
variations were explored using eleven ISSR and nine combinations of SRAP
markers. All markers used generated polymorphic bands among the different
cultivars that can be used as molecular markers for their differentiation.
The genetic distance between cultivars was also estimated from banding
patterns. Our results indicate that ISSR and SRAP systems can efficiently
identify and differentiate between the selected cultivars. This work can be
used as a model to establish a road map for all date palm cultivars
worldwide.
nema |
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| ISSN: | 0534-0012 1820-6069 |
| DOI: | 10.2298/GENSR1601307K |