Effect of Acyl Homoserine Lactone on Recombinant Production of Human Insulin-like Growth Factor-1 in Batch Culture of Escherichia coli

IGF-I as a human growth factor produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa. Up to now, E. coli expression system has been widely used as the host to produce rhIGF-1 with high yields. Acyl Homoserine La...

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Bibliographic Details
Published in:	Protein and peptide letters Vol. 25; no. 11; p. 980
Main Authors:	Babaeipour, Valiollah, Vahidi, Hossein, Alikhani, Shamila, Ranjbari, Javad, Alibakhshi, Abbas, Tabarzad, Maryam
Format:	Journal Article
Language:	English
Published:	Netherlands 01-01-2018
Subjects:	4-Butyrolactone - analogs & derivatives 4-Butyrolactone - pharmacology Cell Proliferation - drug effects Culture Techniques Escherichia coli - cytology Escherichia coli - drug effects Escherichia coli - genetics Humans Insulin-Like Growth Factor I - biosynthesis Insulin-Like Growth Factor I - genetics Quorum Sensing - drug effects Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Transformation, Genetic - drug effects batch culture quorum sensing fermentation origami strain Insulin like growth factor 1 E. coli acyl homoserine lactone
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Summary:	IGF-I as a human growth factor produced in Escherichia coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6 kDa. Up to now, E. coli expression system has been widely used as the host to produce rhIGF-1 with high yields. Acyl Homoserine Lactones (AHLs) are intercellular signaling molecules used in quorum sensing by Gram-negative bacteria. Quorum sensing is a cell density-dependent gene regulation process that allows bacterial cells to express specific genes only when signaling molecules reach the sufficient concentration. For the first time, this study focuses on the N-hexanoyl-L- Homoserine Lactone (HHL) activity on increasing the cell growth and rh-IGF-1concentration in batch culture of E. coli. The maximum production of rhIGF-I was previously optimized in 32y culture medium at 32°C with 0.05 mM IPTG as inducer and 10 g/l glucose concentration. Under this condition, different amounts of HHL (0.001 µg/ml, 1 µg/ml, and 100µg/ml) were evaluated as an inducer for IGF-1 production. Generally, with increasing of HHL concentration, an increase in dry cell weight (2.45 mg/ml to 4.63 mg/ml) and IGF-I expression level (0.4 mg/ml to 0.77 mg/ml) was observed. HHL or other types of AHLs can be considered as protein production inducer in bacterial expression systems through the quorum sensing pathways.
ISSN:	1875-5305
DOI:	10.2174/0929866525666181019150657