HPLC Determination of Imidazoles with Variant Anti-Infective Activity in Their Dosage Forms and Human Plasma

HPLC Determination of Imidazoles with Variant Anti-Infective Activity in Their Dosage Forms and Human Plasma

A suitable HPLC method has been selected and validated for rapid simultaneous separation and determination of four imidazole anti-infective drugs, secnidazole, omeprazole, albendazole, and fenbendazole, in their final dosage forms, in addition to human plasma within 5 min. The method suitability was...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Vol. 26; no. 1; p. 129
Main Authors: Ali, Oday T, Hassan, Wafaa S, Khayyat, Ahdab N, Almalki, Ahmad J, Sebaiy, Mahmoud M
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 30-12-2020
MDPI
Subjects:
Accuracy
Acetonitrile
Albendazole
anti-infective
Antiinfectives and antibacterials
Blood plasma
Chromatography
dosage forms
Drug dosages
Flow velocity
Gastroesophageal reflux
High-performance liquid chromatography
HPLC
human plasma
Imidazole
Infectious diseases
Ions
Liquid chromatography
Methods
Omeprazole
Pharmaceuticals
Phosphoric acid
Plasma
Potassium phosphate
Potassium phosphates
Room temperature
Secnidazole
Separation
Standard deviation
dosage forms
anti-infective
imidazole
HPLC
human plasma
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Summary:A suitable HPLC method has been selected and validated for rapid simultaneous separation and determination of four imidazole anti-infective drugs, secnidazole, omeprazole, albendazole, and fenbendazole, in their final dosage forms, in addition to human plasma within 5 min. The method suitability was derived from the superiority of using the environmentally benign solvent, methanol over acetonitrile as a mobile phase component in respect of safety issues and migration times. Separation of the four anti-infective drugs was performed on a Thermo Scientific BDS Hypersil C column (5 µm, 2.50 × 4.60 mm) using a mobile phase consist of MeOH: 0.025 M KH PO (70:30, ) adjusted to pH 3.20 with ortho-phosphoric acid at room temperature. The flow rate was 1.00 mL/min and maximum absorption was measured with UV detector set at 300 nm. Limits of detection were reported to be 0.41, 0.13, 0.18, and 0.15 µg/mL for secnidazole, omeprazole, albendazole, and fenbendazole, respectively, showing a high degree of the method sensitivity. The method of analysis was validated according to Food and Drug Administration (FDA)guidelines for the determination of the drugs, either in their dosage forms with highly precise recoveries, or clinically in human plasma, especially regarding pharmacokinetic and bioequivalence studies.
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ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26010129