Simultaneous voltammetric detection of 5-hydroxyindole-3-acetic acid and 5-hydroxytryptamine using a glassy carbon electrode modified with conducting polymer and platinised carbon nanofibers

The authors describe a method for simultaneous voltammetric determination of 5-hydroxytryptamine (serotonin; 5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA). A glassy carbon electrode was modified with poly(pyrrole-3-carboxylic acid) and with platinised carbon nanofibers to obtain a sen...

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Bibliographic Details
Published in:	Mikrochimica acta (1966) Vol. 185; no. 9; pp. 412 - 10
Main Authors:	Fredj, Zina, Ali, MounirBen, Singh, Baljit, Dempsey, Eithne
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 01-09-2018 Springer Springer Nature B.V
Subjects:	Acetic acid Analytical Chemistry Carbon fibers Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Conducting polymers Electrodes Glassy carbon Ions Microengineering Nanochemistry Nanofibers Nanotechnology Organic acids Original Paper Polymer industry Polymers Serotonin Silver chloride Urine Voltammetry 5-Hydroxyindole-3-acetic acid 5-Hydroxytryptamine Pyrrole-3-carboxylic acid Stripping square wave voltammetry Pt nanocomposites Quantitation in artificial urine
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Summary:	The authors describe a method for simultaneous voltammetric determination of 5-hydroxytryptamine (serotonin; 5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA). A glassy carbon electrode was modified with poly(pyrrole-3-carboxylic acid) and with platinised carbon nanofibers to obtain a sensor that can quantify 5-HT and 5-HIAA with detection limits of 10 nM and 20 nM, respectively. The peak currents, best measured at voltages of 170 mV and 500 mV (vs. Ag/AgCl) for 5-HT and 5-HIAA, increase linearly in the 0.01–100 μM concentration range for both analytes. The method was successfully applied to the quantitation of 5-HT and 5-HIAA in spiked artificial urine samples, and the sensor can be used up to 10 days. Graphical abstract A new electroanalytical device was developed for separation and quantitation of 5-hydroxytryptamine (5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA), based on stripping square wave voltammetry, exploiting conducting polymer surfaces on platinised carbon nanofiber supports.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0026-3672 1436-5073
DOI:	10.1007/s00604-018-2949-5