Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus

Scientists know very little about the mechanisms underlying fish skin mucus, despite the fact that it is a component of the immune system. Fish skin mucus is an important component of defence against invasive infections. Recently, Fish skin and its mucus are gaining interest among immunologists. Cha...

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Bibliographic Details
Published in:	BMC biotechnology Vol. 24; no. 1; p. 28
Main Authors:	Alabssawy, Ahmed N, Abu-Elghait, Mohammed, Azab, Ahmad M, Khalaf-Allah, Hassan M M, Ashry, Abdelrahman S, Ali, Ahmed O M, Sabra, Abu-Bakr A A, Salem, Salem S
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 04-05-2024 BioMed Central BMC
Subjects:	AgNPs Analysis Animals Anti-Infective Agents - chemistry Anti-Infective Agents - pharmacology Antibiofilm Antibiotics Anticancer properties Antimicrobial Antimicrobial agents Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Bacteria Bacterial infections Biofilms Biofilms - drug effects Biofilms - growth & development Biosynthesis Cancer Candida albicans - drug effects Catfishes Cell Line, Tumor Chlorocebus aethiops Clarias gariepinus Clarias gariepinus catfish Cytotoxicity Epidermis - metabolism Fish Proteins - chemistry Fish Proteins - metabolism Fish Proteins - pharmacology Fish skins Fishes Fungi Gram-positive bacteria Granulation Health aspects Humans Immune system Metal Nanoparticles - chemistry Microbial mats Microbial Sensitivity Tests Microorganisms Mucus Mucus - metabolism Nanoparticles Pathogenic microorganisms Pathogens Physiological aspects Prevention Proteins Pseudomonas aeruginosa - drug effects Silver Silver - chemistry Silver - pharmacology Skin Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Tumor cell lines Vero Cells Wavelength Egypt Japan Antimicrobial AgNPs Cytotoxicity Biosynthesis Antibiofilm Clarias gariepinus catfish
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Summary:	Scientists know very little about the mechanisms underlying fish skin mucus, despite the fact that it is a component of the immune system. Fish skin mucus is an important component of defence against invasive infections. Recently, Fish skin and its mucus are gaining interest among immunologists. Characterization was done on the obtained silver nanoparticles Ag combined with Clarias gariepinus catfish epidermal mucus proteins (EMP-Ag-NPs) through UV-vis, FTIR, XRD, TEM, and SEM. Ag-NPs ranged in size from 4 to 20 nm, spherical in form and the angles were 38.10°, 44.20°, 64.40°, and 77.20°, Where wavelength change after formation of EMP-Ag-NPs as indicate of dark brown, the broad band recorded at wavelength at 391 nm. Additionally, the antimicrobial, antibiofilm and anticancer activities of EMP-Ag-NPs was assessed. The present results demonstrate high activity against unicellular fungi C. albicans, followed by E. faecalis. Antibiofilm results showed strong activity against both S. aureus and P. aeruginosa pathogens in a dose-dependent manner, without affecting planktonic cell growth. Also, cytotoxicity effect was investigated against normal cells (Vero), breast cancer cells (Mcf7) and hepatic carcinoma (HepG2) cell lines at concentrations (200-6.25 µg/mL) and current results showed highly anticancer effect of Ag-NPs at concentrations 100, 5 and 25 µg/mL exhibited rounding, shrinkage, deformation and granulation of Mcf7 and HepG2 with IC50 19.34 and 31.16 µg/mL respectively while Vero cells appeared rounded at concentration 50 µg/mL and normal shape at concentration 25, 12.5 and 6.25 µg/ml with IC50 35.85 µg/mL. This study evidence the potential efficacy of biologically generated Ag-NPs as a substitute medicinal agent against harmful microorganisms. Furthermore, it highlights their inhibitory effect on cancer cell lines.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1472-6750 1472-6750
DOI:	10.1186/s12896-024-00852-7