Reduction of PCR-amplifiable DNA by ethylene oxide treatment of forensic consumables

Highlights • Ethylene oxide treatment of forensic consumables is a powerful method to reduce PCR-amplifiable DNA. • Defined counts of cultured cells can be used to establish treatment conditions. • The amount of reduction in PCR-amplifiable DNA can be monitored using real-time PCR quantification....

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Bibliographic Details
Published in:	Forensic science international : genetics Vol. 12; pp. 185 - 191
Main Authors:	Neureuther, Katharina, Rohmann, Edyta, Hilken, Manuela, Sonntag, Marie-Luise, Herdt, Silke, Koennecke, Thomas, Jacobs, Roland, Adamski, Michalina, Reisbacher, Stefan, Alfs, Knut, Strain, Peter, Bastisch, Ingo
Format:	Journal Article
Language:	English
Published:	Netherlands 01-09-2014
Subjects:	DNA - genetics Ethylene Oxide - chemistry Forensic Genetics Humans Pathology Polymerase Chain Reaction - methods Real-Time Polymerase Chain Reaction Consumables Forensic standard Ethylene oxide Quantification Contamination
Online Access:	Get full text
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Summary:	Highlights • Ethylene oxide treatment of forensic consumables is a powerful method to reduce PCR-amplifiable DNA. • Defined counts of cultured cells can be used to establish treatment conditions. • The amount of reduction in PCR-amplifiable DNA can be monitored using real-time PCR quantification.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1872-4973 1878-0326
DOI:	10.1016/j.fsigen.2014.06.006