Search Results - "Alford, Lea"

Chlamydomonas DYX1C1/PF23 is essential for axonemal assembly and proper morphology of inner dynein arms by Yamamoto, Ryosuke, Obbineni, Jagan M, Alford, Lea M, Ide, Takahiro, Owa, Mikito, Hwang, Juyeon, Kon, Takahide, Inaba, Kazuo, James, Noliyanda, King, Stephen M, Ishikawa, Takashi, Sale, Winfield S, Dutcher, Susan K

“…Cytoplasmic assembly of ciliary dyneins, a process known as preassembly, requires numerous non-dynein proteins, but the identities and functions of these…”
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The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length by Hunter, Emily L, Lechtreck, Karl, Fu, Gang, Hwang, Juyeon, Lin, Huawen, Gokhale, Avanti, Alford, Lea M, Lewis, Brian, Yamamoto, Ryosuke, Kamiya, Ritsu, Yang, Fan, Nicastro, Daniela, Dutcher, Susan K, Wirschell, Maureen, Sale, Winfield S

“…We determined how the ciliary motor I1 dynein is transported. A specialized adapter, IDA3, facilitates I1 dynein attachment to the ciliary transporter called…”
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Chlamydomonas ARMC2/PF27 is an obligate cargo adapter for intraflagellar transport of radial spokes by Lechtreck, Karl F, Liu, Yi, Dai, Jin, Alkhofash, Rama A, Butler, Jack, Alford, Lea, Yang, Pinfen

“…Intraflagellar transport (IFT) carries proteins into flagella but how IFT trains interact with the large number of diverse proteins required to assemble…”
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Assembly of FAP93 at the proximal axoneme in Chlamydomonas cilia by Hwang, Juyeon, Yanagisawa, Haruaki, Davis, Keira C., Hunter, Emily L., Fox, Laura A., Jimenez, Ariana R., Goodwin, Reagan E., Gordon, Sarah A., Stuart, Courtney D. E., Bower, Raqual, Porter, Mary E., Dutcher, Susan K., Sale, Winfield S., Lechtreck, Karl F., Alford, Lea M.

“…To identify proteins specific to the proximal ciliary axoneme, we used iTRAQ to compare short (~2 μm) and full‐length (~11 μm) axonemes of Chlamydomonas. Known…”
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The ciliary inner dynein arm, I1 dynein, is assembled in the cytoplasm and transported by IFT before axonemal docking by Viswanadha, Rasagnya, Hunter, Emily L., Yamamoto, Ryosuke, Wirschell, Maureen, Alford, Lea M., Dutcher, Susan K., Sale, Winfield S.

“…To determine mechanisms of assembly of ciliary dyneins, we focused on the Chlamydomonas inner dynein arm, I1 dynein, also known as dynein f. I1 dynein…”
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Kinesin-13 regulates the quantity and quality of tubulin inside cilia by Vasudevan, Krishna Kumar, Jiang, Yu-Yang, Lechtreck, Karl F, Kushida, Yasuharu, Alford, Lea M, Sale, Winfield S, Hennessey, Todd, Gaertig, Jacek

“…Kinesin-13, an end depolymerizer of cytoplasmic and spindle microtubules, also affects the length of cilia. However, in different models, depletion of…”
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FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c by Vasudevan, Krishna Kumar, Song, Kangkang, Alford, Lea M, Sale, Winfield S, Dymek, Erin E, Smith, Elizabeth F, Hennessey, Todd, Joachimiak, Ewa, Urbanska, Paulina, Wloga, Dorota, Dentler, William, Nicastro, Daniela, Gaertig, Jacek

“…Radial spokes are conserved macromolecular complexes that are essential for ciliary motility. A triplet of three radial spokes, RS1, RS2, and RS3, repeats…”
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The nexin link and B-tubule glutamylation maintain the alignment of outer doublets in the ciliary axoneme by Alford, Lea M., Stoddard, Daniel, Li, Jennifer H., Hunter, Emily L., Tritschler, Douglas, Bower, Raqual, Nicastro, Daniela, Porter, Mary E., Sale, Winfield S.

“…We developed quantitative assays to test the hypothesis that the N‐DRC is required for integrity of the ciliary axoneme. We examined reactivated motility of…”
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Regulation of ciliary motility: Conserved protein kinases and phosphatases are targeted and anchored in the ciliary axoneme by Wirschell, Maureen, Yamamoto, Ryosuke, Alford, Lea, Gokhale, Avanti, Gaillard, Anne, Sale, Winfield S.

“…► Review of regulation of ciliary and flagellar motility by phosphorylation. ► Kinases and phosphatases anchored in the ciliary axoneme. ► Proteomic studies…”
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Analysis of Axonemal Assembly During Ciliary Regeneration in Chlamydomonas by Hunter, Emily L, Sale, Winfield S, Alford, Lea M

“…Chlamydomonas reinhardtii is an outstanding model genetic organism for study of assembly of cilia. Here, methods are described for synchronization of ciliary…”
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Polar expansion during cytokinesis by Gudejko, Heather F.M., Alford, Lea M., Burgess, David R.

“…Vesicle trafficking and new membrane addition at the cleavage furrow have been extensively documented. However, less clear is the old idea that expansion at…”
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The Chlamydomonas mutant pf27 reveals novel features of ciliary radial spoke assembly by Alford, Lea M., Mattheyses, Alexa L., Hunter, Emily L., Lin, Huawen, Dutcher, Susan K., Sale, Winfield S.

“…To address the mechanisms of ciliary radial spoke assembly, we took advantage of the Chlamydomonas pf27 mutant. The radial spokes that assemble in pf27 are…”
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Cytokinesis: A New Lipid Aboard the Raft by Alford, Lea M., Burgess, David R.

“…Several components of membrane rafts play a critical role in cytokinesis. A recent paper reports a new lipid component of these rafts required for proper cell…”
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An Extended Anaphase Signaling Pathway for Mad2p Includes Microtubule Organizing Center Proteins and Multiple Motor-dependent Transitions by Mayer, Christina, Filopei, Jason, Batac, Joe, Alford, Lea, Paluh, Janet L.

“…An Extended Anaphase Signaling Pathway for Mad2p Includes Microtubule Organizing Center Proteins and Multiple Motor-dependent TransitionsSignaling pathways…”
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Cell polarity emerges at first cleavage in sea urchin embryos by Alford, Lea M., Ng, Michelle M., Burgess, David R.

“…In protostomes, cell polarity is present after fertilization whereas most deuterostome embryos show minimal polarity during the early cleavages. We now show…”
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Identification and spatiotemporal control of the asymmetrical membrane cortex in cleavage stage sea urchin embryos by Alford, Lea Marie

“…Polarity established by the first cleavages in sea urchin embryos was investigated in this thesis revealing precocious embryonic polarity. Studies of embryonic…”
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Identification and spatiotemporal control of the asymmetrical membrane cortex in cleavage stage sea urchin embryos by Alford, Lea Marie

“…Polarity established by the first cleavages in sea urchin embryos was investigated in this thesis revealing precocious embryonic polarity. Studies of embryonic…”
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