Search Results - "Alfonzo, Juan D"

Transfer RNA modifications: nature's combinatorial chemistry playground by Jackman, Jane E., Alfonzo, Juan D.

“…Following synthesis, tRNAs are peppered by numerous chemical modifications which may differentially affect a tRNA's structure and function. Although…”
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Do all modifications benefit all tRNAs? by Phizicky, Eric M., Alfonzo, Juan D.

“…Despite the universality of tRNA modifications, some tRNAs lacking specific modifications are subject to degradation pathways, while other tRNAs lacking the…”
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Preferential import of queuosine-modified tRNAs into Trypanosoma brucei mitochondrion is critical for organellar protein synthesis by Kulkarni, Sneha, Rubio, Mary Anne T, Hegedűsová, Eva, Ross, Robert L, Limbach, Patrick A, Alfonzo, Juan D, Paris, Zdeněk

“…Abstract Transfer RNAs (tRNAs) are key players in protein synthesis. To be fully active, tRNAs undergo extensive post-transcriptional modifications, including…”
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Structural basis for sequence-independent substrate selection by eukaryotic wobble base tRNA deaminase ADAT2/3 by Dolce, Luciano G., Zimmer, Aubree A., Tengo, Laura, Weis, Félix, Rubio, Mary Anne T., Alfonzo, Juan D., Kowalinski, Eva

“…The essential deamination of adenosine A 34 to inosine at the wobble base is the individual tRNA modification with the greatest effects on mRNA decoding,…”
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Dynamic queuosine changes in tRNA couple nutrient levels to codon choice in Trypanosoma brucei by Dixit, Sameer, Kessler, Alan C, Henderson, Jeremy, Pan, Xiaobei, Zhao, Ruoxia, D’Almeida, Gabriel Silveira, Kulkarni, Sneha, Rubio, Mary Anne T, Hegedűsová, Eva, Ross, Robert L, Limbach, Patrick A, Green, Brian D, Paris, Zdeněk, Alfonzo, Juan D

“…Abstract Every type of nucleic acid in cells undergoes programmed chemical post-transcriptional modification. Generally, modification enzymes use substrates…”
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Mitochondrial tRNA import--the challenge to understand has just begun by Alfonzo, Juan D, Söll, Dieter

“…Mitochondrial translation is important for the synthesis of proteins involved in oxidative phosphorylation, which yields the bulk of the ATP made in cells…”
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The role of intracellular compartmentalization on tRNA processing and modification by Kessler, Alan C., Silveira d'Almeida, Gabriel, Alfonzo, Juan D.

“…A signature of most eukaryotic cells is the presence of intricate membrane systems. Intracellular organization presumably evolved to provide order, and add…”
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Multi-Substrate Specificity and the Evolutionary Basis for Interdependence in tRNA Editing and Methylation Enzymes by Dixit, Sameer, Henderson, Jeremy C, Alfonzo, Juan D

“…Among tRNA modification enzymes there is a correlation between specificity for multiple tRNA substrates and heteromultimerization. In general, enzymes that…”
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Mammalian mitochondria have the innate ability to import tRNAs by a mechanism distinct from protein import by Rubio, Mary Anne T, Rinehart, Jesse J, Krett, Bethany, Duvezin-Caubet, Stéphane, Reichert, Andreas S, Söll, Dieter, Alfonzo, Juan D

“…Mitochondrial genomes generally encode a minimal set of tRNAs necessary for protein synthesis. However, a number of eukaryotes import tRNAs from the cytoplasm…”
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Post-transcriptional modifications are very important after all by Alfonzo, Juan D

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Unusual Noncanonical Intron Editing Is Important for tRNA Splicing in Trypanosoma brucei by Rubio, Mary Anne T, Paris, Zdeněk, Gaston, Kirk W, Fleming, Ian M.C, Sample, Paul, Trotta, Christopher R, Alfonzo, Juan D

“…In cells, tRNAs are synthesized as precursor molecules bearing extra sequences at their 5′ and 3′ ends. Some tRNAs also contain introns, which, in archaea and…”
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TbUTP10, a protein involved in early stages of pre-18S rRNA processing in Trypanosoma brucei by Faktorová, Drahomíra, Bär, Anita, Hashimi, Hassan, McKenney, Katherine, Horák, Aleš, Schnaufer, Achim, Rubio, Mary Anne T., Alfonzo, Juan D., Lukeš, Julius

“…[Display omitted] •Identification and characterization of the essential nucleolar protein TbUTP10 in Trypanosoma brucei.•TbUTP10 is a homolog of yeast U3 small…”
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Identification of 2-methylthio cyclic N6-threonylcarbamoyladenosine (ms2ct6A) as a novel RNA modification at position 37 of tRNAs by Kang, Byeong-Il, Miyauchi, Kenjyo, Matuszewski, Michal, D'Almeida, Gabriel Silveira, Rubio, Mary Anne T, Alfonzo, Juan D, Inoue, Kazuki, Sakaguchi, Yuriko, Suzuki, Takeo, Sochacka, Elzbieta, Suzuki, Tsutomu

“…Transfer RNA modifications play pivotal roles in protein synthesis. N6-threonylcarbamoyladenosine (t6A) and its derivatives are modifications found at position…”
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METTLing in the right place: METTL8 is a mitochondrial tRNA-specific methyltransferase by Kowalinski, Eva, Alfonzo, Juan D

“…Schöller et al. (2021) discovered that METTL8, thought of as an mRNA modifier, is a tRNA-specific mitochondrial enzyme important for mitochondrial translation…”
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Posttranscriptional RNA Modifications: Playing Metabolic Games in a Cell’s Chemical Legoland by Helm, Mark, Alfonzo, Juan D.

“…Nature combines existing biochemical building blocks, at times with subtlety of purpose. RNA modifications are a prime example of this, where standard RNA…”
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The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr by Lopes, Raphael R S, Silveira, Gilbert de O, Eitler, Roberta, Vidal, Raphael S, Kessler, Alan, Hinger, Scott, Paris, Zdeněk, Alfonzo, Juan D, Polycarpo, Carla

“…Trypanosoma brucei, the etiologic agent of sleeping sickness, encodes a single intron-containing tRNA, tRNA(Tyr), and splicing is essential for its viability…”
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From Prebiotics to Probiotics: The Evolution and Functions of tRNA Modifications by McKenney, Katherine M, Alfonzo, Juan D

“…All nucleic acids in cells are subject to post-transcriptional chemical modifications. These are catalyzed by a myriad of enzymes with exquisite specificity…”
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Next steps in transcriptomics: Understanding RNA modifications at a genomic scale by Accornero, Federica, Alfonzo, Juan D.

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Thiolation Controls Cytoplasmic tRNA Stability and Acts as a Negative Determinant for tRNA Editing in Mitochondria by Wohlgamuth-Benedum, Jessica M., Rubio, Mary Anne T., Paris, Zdeněk, Long, Shaojun, Poliak, Pavel, Lukeš, Julius, Alfonzo, Juan D.

“…Kinetoplastids encode a single nuclear tryptophanyl tRNA that contains a CCA anticodon able to decode the UGG codons used in cytoplasmic protein synthesis but…”
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adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA by Rubio, Mary Anne T, Pastar, Irena, Gaston, Kirk W, Ragone, Frank L, Janzen, Christian J, Cross, George A.M, Papavasiliou, F. Nina, Alfonzo, Juan D

“…Adenosine-to-inosine editing in the anticodon of tRNAs is essential for viability. Enzymes mediating tRNA adenosine deamination in bacteria and yeast contain…”
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