Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies

Antifungal susceptibility testing of Malassezia yeast: comparison of two different methodologies

Summary All Malassezia species are lipophilic; thus, modifications are required in susceptibility testing methods to ensure their growth. Antifungal susceptibility of Malassezia species using agar and broth dilution methods has been studied. Currently, few tests using disc diffusion methods are bein...

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Bibliographic Details
Published in:Mycoses Vol. 60; no. 2; pp. 104 - 111
Main Authors: Rojas, Florencia D., Córdoba, Susana B., Ángeles Sosa, María, Zalazar, Laura C., Fernández, Mariana S., Cattana, María E., Alegre, Liliana R., Carrillo‐Muñoz, Alfonso J., Giusiano, Gustavo E.
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 01-02-2017
Subjects:
Agar
Agar diffusion test
Amphotericin B
Amphotericin B - pharmacology
Antifungal agents
Antifungal Agents - pharmacology
Culture Media
Diffusion
disc diffusion
Fluconazole
Fluconazole - pharmacology
Itraconazole
Itraconazole - pharmacology
lipid‐dependent yeast
Lipophilic
Malassezia
Malassezia - drug effects
Malassezia - growth & development
Miconazole
Miconazole - pharmacology
Microbial Sensitivity Tests - methods
minimal inhibitory concentration
Minimum inhibitory concentration
Tablets
Voriconazole
Voriconazole - pharmacology
Yeast
lipid-dependent yeast
minimal inhibitory concentration
disc diffusion
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Summary:Summary All Malassezia species are lipophilic; thus, modifications are required in susceptibility testing methods to ensure their growth. Antifungal susceptibility of Malassezia species using agar and broth dilution methods has been studied. Currently, few tests using disc diffusion methods are being performed. The aim was to evaluate the in vitro susceptibility of Malassezia yeast against antifungal agents using broth microdilution and disc diffusion methods, then to compare both methodologies. Fifty Malassezia isolates were studied. Microdilution method was performed as described in reference document and agar diffusion test was performed using antifungal tablets and discs. To support growth, culture media were supplemented. To correlate methods, linear regression analysis and categorical agreement was determined. The strongest linear association was observed for fluconazole and miconazole. The highest agreement between both methods was observed for itraconazole and voriconazole and the lowest for amphotericin B and fluconazole. Although modifications made to disc diffusion method allowed to obtain susceptibility data for Malassezia yeast, variables cannot be associated through a linear correlation model, indicating that inhibition zone values cannot predict MIC value. According to the results, disc diffusion assay may not represent an alternative to determine antifungal susceptibility of Malassezia yeast.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0933-7407
1439-0507
DOI:10.1111/myc.12556