Cdc15 is required for spore morphogenesis independently of Cdc14 in Saccharomyces cerevisiae

In Saccharomyces cerevisiae exit from mitosis requires the Cdc14 phosphatase to reverse CDK-mediated phosphorylation. Cdc14 is released from the nucleolus by the Cdc14 early anaphase release (FEAR) and mitotic exit network (MEN) pathways. In meiosis, the FEAR pathway is essential for exit from anaph...

Full description

Saved in:

Bibliographic Details
Published in:	Genetics (Austin) Vol. 177; no. 1; pp. 281 - 293
Main Authors:	Pablo-Hernando, M.E, Arnaiz-Pita, Y, Nakanishi, H, Dawson, D, Rey, F. del, Neiman, A.M, Aldana, C.R.V. de
Format:	Journal Article
Language:	English
Published:	United States Genetics Soc America 01-09-2007 Genetics Society of America Copyright © 2007 by the Genetics Society of America
Subjects:	Active Transport, Cell Nucleus ascospores Cdc15 protein Cell cycle Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Cell division Cell Nucleolus fungal proteins Genetics GTP-Binding Proteins - genetics GTP-Binding Proteins - metabolism Investigations meiosis Meiosis - physiology morphogenesis Mutation phosphoprotein phosphatase prospore membrane Protein Tyrosine Phosphatases - genetics Protein Tyrosine Phosphatases - metabolism Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism septins Signal transduction spindle pole body Spores, Fungal - physiology sporulation Yeast
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	In Saccharomyces cerevisiae exit from mitosis requires the Cdc14 phosphatase to reverse CDK-mediated phosphorylation. Cdc14 is released from the nucleolus by the Cdc14 early anaphase release (FEAR) and mitotic exit network (MEN) pathways. In meiosis, the FEAR pathway is essential for exit from anaphase I. The MEN component Cdc15 is required for the formation of mature spores. To analyze the role of Cdc15 during sporulation, a conditional mutant in which CDC15 expression was controlled by the CLB2 promoter was used. Cdc15-depleted cells proceeded normally through the meiotic divisions but were unable to properly disassemble meiosis II spindles. The morphology of the prospore membrane was aberrant and failed to capture the nuclear lobes. Cdc15 was not required for Cdc14 release from the nucleoli, but it was essential to maintain Cdc14 released and for its nucleo-cytoplasmic transport. However, cells carrying a CDC14 allele with defects in nuclear export (Cdc14-DeltaNES) were able to disassemble the spindle and to complete spore formation, suggesting that the Cdc14 nuclear export defect was not the cause of the phenotypes observed in cdc15 mutants.
Bibliography:	http://www.genetics.org/ ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Corresponding author: Instituto de Microbiología Bioquímica, Departamento de Microbiología y Genética, CSIC/Universidad de Salamanca, 37007 Salamanca, Spain. E-mail: cvazquez@usal.es. Communicating editor: A. P. Mitchell
ISSN:	0016-6731 1943-2631 1943-2631
DOI:	10.1534/genetics.107.076133