Search Results - "Alcolea, Pedro J."

Functional genomics in sand fly-derived Leishmania promastigotes by Alcolea, Pedro J, Alonso, Ana, Molina, Ricardo, Jiménez, Maribel, Myler, Peter J, Larraga, Vicente

“…Leishmania development in the sand fly gut leads to highly infective forms called metacyclic promastigotes. This process can be routinely mimicked in culture…”
Get full text

An Insight into the proteome of Crithidia fasciculata choanomastigotes as a comparative approach to axenic growth, peanut lectin agglutination and differentiation of Leishmania spp. promastigotes by Alcolea, Pedro J, Alonso, Ana, García-Tabares, Francisco, Toraño, Alfredo, Larraga, Vicente

“…The life cycle of the trypanosomatid Crithidia fasciculata is monogenetic, as the unique hosts of these parasites are different species of culicids. The…”
Get full text

IL12 p35 and p40 subunit genes administered as pPAL plasmid constructs do not improve protection of pPAL-LACK vaccine against canine leishmaniasis by Alcolea, Pedro J, Alonso, Ana, Esteban, Adriana, Peris, Paz, Cortés, Alberto, Castillo, Juan A, Larraga, Vicente

“…Leishmania infantum causes zoonotic visceral leishmaniasis (ZVL) in the Mediterranean basin and South America. The parasite has been shown to co-infect HIV…”
Get full text

Influence of the Microenvironment in the Transcriptome of Leishmania infantum Promastigotes: Sand Fly versus Culture by Alcolea, Pedro J, Alonso, Ana, Domínguez, Mercedes, Parro, Víctor, Jiménez, Maribel, Molina, Ricardo, Larraga, Vicente

“…Zoonotic visceral leishmaniasis is a vector-borne disease caused by Leishmania infantum in the Mediterranean Basin, where domestic dogs and wild canids are the…”
Get full text

Increased Abundance of Proteins Involved in Resistance to Oxidative and Nitrosative Stress at the Last Stages of Growth and Development of Leishmania amazonensis Promastigotes Revealed by Proteome Analysis by Alcolea, Pedro J, Alonso, Ana, García-Tabares, Francisco, Mena, María C, Ciordia, Sergio, Larraga, Vicente

“…Leishmania amazonensis is one of the major etiological agents of the neglected, stigmatizing disease termed american cutaneous leishmaniasis (ACL). ACL is a…”
Get full text

Non-replicative antibiotic resistance-free DNA vaccine encoding S and N proteins induces full protection in mice against SARS-CoV-2 by Alcolea, Pedro J., Larraga, Jaime, Rodríguez-Martín, Daniel, Alonso, Ana, Loayza, Francisco J., Rojas, José M., Ruiz-García, Silvia, Louloudes-Lázaro, Andrés, Carlón, Ana B., Sánchez-Cordón, Pedro J., Nogales-Altozano, Pablo, Redondo, Natalia, Manzano, Miguel, Lozano, Daniel, Palomero, Jesús, Montoya, María, Vallet-Regí, María, Martín, Verónica, Sevilla, Noemí, Larraga, Vicente

“…SARS-CoV-2 vaccines currently in use have contributed to controlling the COVID-19 pandemic. Notwithstanding, the high mutation rate, fundamentally in the spike…”
Get full text

Serum Removal from Culture Induces Growth Arrest, Ploidy Alteration, Decrease in Infectivity and Differential Expression of Crucial Genes in Leishmania infantum Promastigotes by Alcolea, Pedro J, Alonso, Ana, Moreno-Izquierdo, Miguel A, Degayón, María A, Moreno, Inmaculada, Larraga, Vicente

“…Leishmania infantum is one of the species responsible for visceral leishmaniasis. This species is distributed basically in the Mediterranean basin. A recent…”
Get full text

Leishmania infantum UBC1 in Metacyclic Promastigotes from Phlebotomus perniciosus , a Vaccine Candidate for Zoonotic Visceral Leishmaniasis by Larraga, Jaime, Alcolea, Pedro J, Alonso, Ana M, Martins, Luis T C, Moreno, Inmaculada, Domínguez, Mercedes, Larraga, Vicente

“…parasites cause outstanding levels of morbidity and mortality in many developing countries in tropical and subtropical regions. Numerous gene expression…”
Get full text

RNA-seq analysis reveals differences in transcript abundance between cultured and sand fly-derived Leishmania infantum promastigotes by Alcolea, Pedro J., Alonso, Ana, Baugh, Loren, Paisie, Carolyn, Ramasamy, Gowthaman, Sekar, Aarthi, Sur, Aakash, Jiménez, Maribel, Molina, Ricardo, Larraga, Vicente, Myler, Peter J.

“…Leishmania infantum is responsible for human and canine leishmaniasis in the Mediterranean basin, where the major vector is Phlebotomus perniciosus. Because…”
Get full text

Temperature increase prevails over acidification in gene expression modulation of amastigote differentiation in Leishmania infantum by Alcolea, Pedro J, Alonso, Ana, Gómez, Manuel J, Sánchez-Gorostiaga, Alicia, Moreno-Paz, Mercedes, González-Pastor, Eduardo, Toraño, Alfredo, Parro, Víctor, Larraga, Vicente

“…The extracellular promastigote and the intracellular amastigote stages alternate in the digenetic life cycle of the trypanosomatid parasite Leishmania…”
Get full text

Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening by San Martin-Uriz, Patxi, Mirete, Salvador, Alcolea, Pedro J, Gomez, Manuel J, Amils, Ricardo, Gonzalez-Pastor, Jose E

“…Acidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metal-resistant organisms; yet little is known…”
Get full text

The contribution of DNA microarray technology to gene expression profiling in Leishmania spp.: A retrospective view by Alonso, Ana, Larraga, Vicente, Alcolea, Pedro J.

“…The first completed genome project of any living organism, excluding viruses, was of the gammaproteobacteria Haemophilus influenzae in 1995. Until the last…”
Get full text

Rationale for selection of developmentally regulated genes as vaccine candidates against Leishmania infantum infection by Alcolea, Pedro J, Alonso, Ana, Larraga, Vicente

“…[...]genes up-regulated in the infective stages of the parasite code potential vaccine candidates, which has been considered herein together with biological…”
Get full text

The antibiotic resistance-free mammalian expression plasmid vector pPAL for development of third generation vaccines by Alcolea, Pedro J., Alonso, Ana, Larraga, Vicente

“…DNA vaccines require a vector to replicate genes and express encoding antigens. Antibiotic resistance genes are often used as selection markers, which must not…”
Get full text

Guide RNA genes up-regulated in Leishmania infantum metacyclic promastigotes by Alcolea, Pedro J., Alonso, Ana, Larraga, Vicente

“…The kinetoplastid parasite Leishmania infantum is responsible for zoonotic visceral leishmaniasis in the mediterranean basin, where dogs are the reservoir…”
Get full text

Quantitative RNA Analysis Using RNA-Seq by Myler, Peter J, McDonald, Jacqueline A, Alcolea, Pedro J, Sur, Aakash

“…High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital readout of mRNA levels over several orders of magnitude, as well as mapping the…”
Get more information

An insight into differential protein abundance throughout Leishmania donovani promastigote growth and differentiation by Alcolea, Pedro J., Alonso, Ana, García-Tabares, Francisco, Larraga, Jaime, Martins, Luis T. C., Loayza, Franciso J., Ruiz-García, Silvia, Larraga, Vicente

“…Leishmania donovani causes anthroponotic visceral leishmaniasis, responsible for about 50,000 annual deaths worldwide. Current therapies have considerable side…”
Get full text

Proteome Profiling of Leishmania Infantum Promastigotes by ALCOLEA, PEDRO J, ALONSO, ANA, LARRAGA, VICENTE

“…A proteome analysis of the promastigote stage of the trypanosomatid parasite Leishmania infantum (MON-1 zymodeme) is described here for the first time. Total…”
Get full text

In vitro infectivity and differential gene expression of Leishmania infantum metacyclic promastigotes: negative selection with peanut agglutinin in culture versus isolation from the stomodeal valve of Phlebotomus perniciosus by Alcolea, Pedro J, Alonso, Ana, Degayón, María A, Moreno-Paz, Mercedes, Jiménez, Maribel, Molina, Ricardo, Larraga, Vicente

“…Leishmania infantum is the protozoan parasite responsible for zoonotic visceral leishmaniasis in the Mediterranean basin. A recent outbreak in humans has been…”
Get full text

An Insight into the Constitutive Proteome Throughout Leishmania donovani Promastigote Growth and Differentiation by Alcolea, Pedro J., Alonso, Ana, García-Tabares, Francisco, Mena, María del Carmen, Ciordia, Sergio, Larraga, Vicente

“…Anthroponotic visceral leishmaniasis is a life-threatening disease caused by Leishmania donovani (Kinetoplastida: Trypanosomatidae) in East Africa and the…”
Get full text