Search Results - "Alberts, B M"

Head-On Collision Between a DNA Replication Apparatus and RNA Polymerase Transcription Complex by Liu, Bin, Alberts, Bruce M.

“…An in vitro system reconstituted from purified proteins has been used to examine what happens when the DNA replication apparatus of bacteriophage T4 collides…”
Get full text

Reorganization of the cytoskeleton during Drosophila oogenesis: implications for axis specification and intercellular transport by THEURKAUF, W. E, SMILEY, S, MEI LIE WONG, ALBERTS, B. M

“…Inhibitor studies have implicated microtubules in at least three important developmental processes during Drosophila oogenesis: oocyte determination and growth…”
Get full text

Organization of the Cytoskeleton in Early Drosophila Embryos by Karr, Timothy L., Alberts, Bruce M.

“…The cytoskeleton of early, non-cellularized Drosophila embryos has been examined by indirect immunofluorescence techniques, using whole mounts to visualize the…”
Get full text

A central role for microtubules in the differentiation of Drosophila oocytes by THEURKAUF, W. E, ALBERTS, B. M, YUH NUNG JAN, JONGENS, T. A

“…Drosophila oocytes develop within cysts containing 16 cells that are interconnected by cytoplasmic bridges. Although the cysts are syncytial, the 16 cells…”
Get full text

Actin-Binding Proteins from Drosophila Embryos: A Complex Network of Interacting Proteins Detected by F-Actin Affinity Chromatography by Miller, Kathryn G., Field, Christine M., Alberts, Bruce M.

“…By using F-actin affinity chromatography columns to select proteins solely by their ability to bind to actin filaments, we have identified and partially…”
Get full text

Studies of nuclear and cytoplasmic behaviour during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis by FOE, V. E, ALBERTS, B. M

“…Using differential interference contrast optics, combined with cinematography, we have studied the morphological changes that the living, syncytial embryo…”
Get full text

Identification of Microtubule-Associated Proteins in the Centrosome, Spindle, and Kinetochore of the Early Drosophila Embryo by Kellogg, Douglas R., Field, Christine M., Alberts, Bruce M.

“…We have developed affinity chromatography methods for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts and have used…”
Get full text

Prokaryotic DNA Replication Mechanisms by Alberts, B. M.

“…The three different prokaryotic replication systems that have been most extensively studied use the same basic components for moving a DNA replication fork,…”
Get full text

The rapid dissociation of the T4 DNA polymerase holoenzyme when stopped by a DNA hairpin helix. A model for polymerase release following the termination of each Okazaki fragment by Hacker, K J, Alberts, B M

“…We have examined the molecular mechanism that enables the T4 bacteriophage DNA polymerase holoenzyme to synthesize DNA processively on the leading strand of…”
Get full text

Purification and characterization of the T4 bacteriophage uvsX protein by Formosa, T, Alberts, B M

“…Gene uvsX of bacteriophage T4 encodes a 40,000-dalton protein that plays a key role in the major pathway for genetic recombination in T4-infected cells…”
Get full text

DNA synthesis dependent on genetic recombination: characterization of a reaction catalyzed by purified bacteriophage T4 proteins by Formosa, T, Alberts, B M

“…To simulate a reaction that occurs in T4-infected cells, we have developed an in vitro DNA synthesis system that requires seven highly purified proteins…”
Get more information

The DNA replication fork can pass RNA polymerase without displacing the nascent transcript by Liu, Bin, Lie Wong, Mei, Tinker, Rachel L, Geiduschek, E. Peter, Alberts, Bruce M

“…Replication proteins encoded by bacteriophage T4 generate DNA replication forks that can pass a molecule of Escherichia coli RNA polymerase moving in the same…”
Get full text

The T4 DNA polymerase accessory proteins form an ATP-dependent complex on a primer-template junction by MUNN, M. M, ALBERTS, B. M

“…The DNA polymerase holoenzyme of bacteriophage T4 contains, besides the DNA polymerase itself (the gene 43 protein), a complex of the protein products of T4…”
Get full text

DNA footprinting studies of the complex formed by the T4 DNA polymerase holoenzyme at a primer-template junction by MUNN, M. M, ALBERTS, B. M

“…We have used DNA footprinting techniques to analyze the interactions of five DNA replication proteins at a primer-template junction: the bacteriophage T4 DNA…”
Get full text

The bacteriophage T4 DNA replication fork. Only DNA helicase is required for leading strand DNA synthesis by the DNA polymerase holoenzyme by Cha, T A, Alberts, B M

“…Seven bacteriophage T4-encoded proteins reconstitute a DNA replication apparatus that catalyzes coupled leading and lagging strand DNA synthesis at a…”
Get full text

Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos by Minden, Jonathan S., Agard, David A., Sedat, John W., Alberts, Bruce M.

“…One of the first signs of cell differentiation in the Drosophila melanogaster embryo occurs 3 h after fertilization, when discrete groups of cells enter their…”
Get full text

Reversible Chromosome Condensation Induced in Drosophila Embryos by Anoxia: Visualization of Interphase Nuclear Organization by Foe, Victoria E., Alberts, Bruce M.

“…We have studied the morphology of nuclei in Drosophila embryos during the syncytial blastoderm stages. Nuclei in living embryos were viewed with differential…”
Get full text

The bacteriophage T4 insertion/substitution vector system. A method for introducing site-specific mutations into the virus chromosome by Selick, H E, Kreuzer, K N, Alberts, B M

“…A bacteriophage T4 insertion/substitution vector system has been developed as a means of introducing in vitro generated mutations into the T4 chromosome. The…”
Get full text

The mechanism of homologous DNA strand exchange catalyzed by the bacteriophage T4 uvsX and gene 32 proteins by Kodadek, T, Wong, M L, Alberts, B M

“…A strand exchange reaction between a single-stranded DNA circle and a homologous linear double-stranded DNA molecule is catalyzed by a mixture of two T4…”
Get full text

Studies of the DNA helicase-RNA primase unit from bacteriophage T4. A trinucleotide sequence on the DNA template starts RNA primer synthesis by Cha, T A, Alberts, B M

“…The purified DNA replication proteins encoded by genes 41 and 61 of bacteriophage T4 catalyze efficient RNA primer synthesis on a single-stranded DNA template…”
Get full text