Detection and spread of oxa-48-producing Klebsiella oxytoca isolates in Istanbul, Turkey

Five OXA-48 producing Klebsiella oxytoca strains isolated in April-July 2010 were analyzed. Antibiotic susceptibility tests were performed using disc diffusion method and VITEK 2 system. Carbapenemase activity was investigated using the Modified Hodge test. Beta-lactamase genes were detected by PCR...

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Published in:	Southeast Asian journal of tropical medicine and public health Vol. 45; no. 1; pp. 123 - 129
Main Authors:	Nazik, Hasan, Aydin, Selda, Albayrak, Rüveyda, Bilgi, Esma A, Yildiz, Ismail, Kuvat, Nuray, Kelesoglu, Fatih M, Pakaştiçali, Nagehan, Yilmaz, Fadime, Ongen, Betigül
Format:	Journal Article
Language:	English
Published:	Thailand Central Coordinating Board, SEAMEO-TROPMED Project 01-01-2014
Subjects:	Anti-Bacterial Agents - pharmacology Carbapenems - pharmacology Disease Outbreaks Electrophoresis, Gel, Pulsed-Field Female Humans Infant Klebsiella Infections - drug therapy Klebsiella Infections - epidemiology Klebsiella Infections - microbiology Klebsiella Infections - transmission Klebsiella oxytoca Klebsiella oxytoca - drug effects Klebsiella oxytoca - enzymology Klebsiella oxytoca - genetics Klebsiella oxytoca - isolation & purification Male Microbial Sensitivity Tests Polymerase Chain Reaction Treatment Outcome Turkey Turkey
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Summary:	Five OXA-48 producing Klebsiella oxytoca strains isolated in April-July 2010 were analyzed. Antibiotic susceptibility tests were performed using disc diffusion method and VITEK 2 system. Carbapenemase activity was investigated using the Modified Hodge test. Beta-lactamase genes were detected by PCR and blaOXA-48 was sequenced. Genetic relatedness between K. oxytoca isolates was investigated by pulse-field gel electrophoresis (PFGE). Carbapenemase activity was detected in 5 isolates by Modified Hodge test. Although all strains were resistant to ertapenem and imipenem, only one strain was also resistant to meropenem. BlaOXA-48 in 4 isolates harbored 2 or 3 other ESBL types, namely, blaTEM, blaSHV, blaCTX-M, or blaVEB. PFGE revealed 3 different pulso-types among the K. oxytoca isolates. The presence of OXA-48 carbapenemase in other species of clinical isolates should also be considered.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0125-1562 0038-3619