Differentiation of Bone Marrow: Derived Mesenchymal Stem Cells into Hepatocyte-like Cells

Differentiation of Bone Marrow: Derived Mesenchymal Stem Cells into Hepatocyte-like Cells

Cirrhosis is the end-stage liver fibrosis, whereby normal liver architecture is disrupted by fibrotic bands, parenchymal nodules and vascular distortion. Portal hypertension and hepatocyte dysfunction are the end results and give rise to major systemic complications and premature death. Mesenchymal...

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Published in:Indian journal of hematology & blood transfusion Vol. 32; no. 3; pp. 276 - 283
Main Authors: Al Ghrbawy, Nesrien M., Afify, Reham Abdel Aleem Mohamed, Dyaa, Nehal, El Sayed, Asmaa A.
Format: Journal Article
Language:English
Published: New Delhi Springer India 01-09-2016
Springer Nature B.V
Subjects:
Blood Transfusion Medicine
Bone marrow
Cell growth
Growth factors
Hematology
Human Genetics
Liver
Medicine
Medicine & Public Health
Oncology
Original
Original Article
Protein expression
Stem cells
Hepatocyte
Differentiation
Growth factors
Mesenchymal stem cells
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Summary:Cirrhosis is the end-stage liver fibrosis, whereby normal liver architecture is disrupted by fibrotic bands, parenchymal nodules and vascular distortion. Portal hypertension and hepatocyte dysfunction are the end results and give rise to major systemic complications and premature death. Mesenchymal stem cells (MSC) have the capacity of self-renew and to give rise to cells of various lineages, so MSC can be isolated from bone marrow (BM) and induced to differentiate into hepatocyte-like cells. MSC were induced to differentiate into hepatocyte-like cells by hepatotic growth factor (HGF) and fibroblast growth factor-4 (FGF-4). Differentiated cells were examined for the expression of hepatocyte-specific markers and hepatocyte functions. MSC were isolated. Flow cytometry analysis showed that they expressed the MSC-specific markers, reverse transcriptase–polymerase chain reaction (RT-PCR) demonstrated that MSC expressed the hepatocyte-specific marker cytokeratin 18 (CK-18) following hepatocyte induction. This study demonstrates that BM-derived—MSC can differentiate into functional hepatocyte-like cells following the induction of HGF and FGF-4. MSC can serve as a favorable cell source for tissue engineering in the treatment of liver disease.
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ISSN:0971-4502
0974-0449
0974-0449
0971-4502
DOI:10.1007/s12288-015-0581-7