Effects of Extraction Methods and Conditions on Bioactive Compounds Extracted from Phaeodactylum tricornutum

The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective param...

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Bibliographic Details
Published in:	Acta chimica Slovenica Vol. 67; no. 4; pp. 1250 - 1261
Main Authors:	AkyÄ L, Saniye, Ä Lter, IĹźÄ L, KoĂ, Mehmet, Demirel, Zeliha, ErdoÄźan, AyĹźegĂĽl, Dalay, Meltem Conk, Ertekin, Figen Kaymak
Format:	Journal Article
Language:	English
Published:	Slovenia Slovenian Chemical Society 01-12-2020
Subjects:	fucoxanthin microwave extraction optimization phaeodactylum tricornutum total phenolic compounds ultrasonic extraction
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Summary:	The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective parameter on fucoxanthin content, total phenolic content and antioxidant activity. The maximum fucoxanthin content (5.60 ± 0.06 mg/g) and antioxidant activity (763.00 ± 15.88 EC50 ?g/mL extract) were obtained with the homogenization extraction method whose optimum conditions were 1.93% biomass/solvent ratio, ~5200 rpm homogenization rate and 14.2 min extraction time. Although the ultrasonic extraction method has reached the approximately same level of fucoxanthin content (5.24 ± 0.07 mg/g)), TPC (67.68 ± 1.58 mg gallic acid/L) and antioxidant activity (619.90 ± 17.16 EC50 ?g/mL extract) at an amplitude of 55.72%, a higher biomass/ solvent ratio (2.72%) and a longer extraction time (17.37 min) have been required. The lowest fucoxanthin content, total phenolic content and antioxidant activity were determined for the microwave extraction method.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1580-3155 1318-0207 1580-3155
DOI:	10.17344/acsi.2020.6157