REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS

REGULATORY MECHANISMS OF HUMAN MONOCYTIC THP-1 CELLS TRANSENDOTHELIAL MIGRATION INDUCED BY STREPTOCOCCUS PYOGENES COMPONENTS

Enhanced transendothelial migration of leukocytes during infection and inflammation occurs due to increased expression of ICAM-1 and VCAM-1 adherence molecules of the immunoglobulin superfamily, and increased secretion of chemokines by endothelial cells. Our previous in vitro studies with human mono...

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Bibliographic Details
Published in:Medit͡s︡inskai͡a︡ immunologii͡a Vol. 15; no. 5; pp. 457 - 464
Main Authors: Starikova, E. A., Lebedeva, A. M., Aktureeva, N. A., Burova, L. A., Freidlin, I. S.
Format: Journal Article
Language:English
Russian
Published: St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists 25-07-2014
Subjects:
streptococcus pyogenes
thp-1 cells
transendothelial migration
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Summary:Enhanced transendothelial migration of leukocytes during infection and inflammation occurs due to increased expression of ICAM-1 and VCAM-1 adherence molecules of the immunoglobulin superfamily, and increased secretion of chemokines by endothelial cells. Our previous in vitro studies with human monocytic THP-1 cells showed that some components of Streptococcus pyogenes caused a sufficient enhancement of transendothelial cell migration. However, expression levels of adhesion molecules remained unchanged on endothelial cells. The purpose of the work was to study mechanisms underlying the enhanced transendothelial migration of THP-1 cells treated with supernatante of streptococcal cells (SSC). SSC exhibited chemoattractive activity towards THP-1 cells. SSC increased chemotaxis and transmigration of THP-1 cells. Meanwhile, a 24-hour preincubation of THP-1 cells with SSC caused suppression of the both effects, being accompanied by decreased level of Focal Adhesion Kinase phosphorylation. Preincubation of THP-1 cells with pertussis toxin did reduce the transmigration intensity in presence of the SSC down to the level of spontaneous migration. These results suggest that increased transmigration of THP-1 cells under the influence of SSC may be determined by its chemoattractive action upon the migrating cells.
ISSN:1563-0625
2313-741X
DOI:10.15789/1563-0625-2013-5-457-464