Gold-Modified Micellar Composites as Colorimetric Probes for the Determination of Low Molecular Weight Thiols in Biological Fluids Using Consumer Electronic Devices

Gold-Modified Micellar Composites as Colorimetric Probes for the Determination of Low Molecular Weight Thiols in Biological Fluids Using Consumer Electronic Devices

This work describes a new, low-cost and simple-to-use method for the determination of free biothiols in biological fluids. The developed method utilizes the interaction of biothiols with gold ions, previously anchored on micellar assemblies through electrostatic interactions with the hydrophilic hea...

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Bibliographic Details
Published in:Applied sciences Vol. 11; no. 6; p. 2705
Main Authors: Akrivi, Elli A., Vlessidis, Athanasios G., Giokas, Dimosthenis L., Kourkoumelis, Nikolaos
Format: Journal Article
Language:English
Published: Basel MDPI AG 01-03-2021
Subjects:
Alzheimer's disease
Ammonium
Ammonium bromides
Anions
biothiols
Blood plasma
Calibration
Cations
Chloride
Color
Coloration
colorimetric probes
Colorimetry
Computational fluid dynamics
Electronic equipment
Electrostatic properties
Flatbed
Gold
gold nanoparticles
Homocysteine
Ions
Laboratories
Low molecular weights
Micelles
Molecular weight
Nanomaterials
Nanoparticles
Plasma
Proteins
Quenching
Scanners
Sodium
Substitution reactions
Surfactants
Thiols
Urine
United States > US
Germany
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Summary:This work describes a new, low-cost and simple-to-use method for the determination of free biothiols in biological fluids. The developed method utilizes the interaction of biothiols with gold ions, previously anchored on micellar assemblies through electrostatic interactions with the hydrophilic headgroup of cationic surfactant micelles. Specifically, the reaction of AuCl4− with the cationic surfactant cetyltrimethyl ammonium bromide (CTAB) produces an intense orange coloration, due to the ligand substitution reaction of the Br− for Cl− anions, followed by the coordination of the AuBr4− anions on the micelle surface through electrostatic interactions. When biothiols are added to the solution, they complex with the gold ions and disrupt the AuBr4−–CTAB complex, quenching the initial coloration and inducing a decrease in the light absorbance of the solution. Biothiols are assessed by monitoring their color quenching in an RGB color model, using a flatbed scanner operating in transmittance mode as an inexpensive microtiter plate photometer. The method was applied to determine the biothiol content in urine and blood plasma samples, with satisfactory recoveries (i.e., >67.3–123% using external calibration and 103.8–115% using standard addition calibration) and good reproducibility (RSD < 8.4%, n = 3).
ISSN:2076-3417
2076-3417
DOI:10.3390/app11062705