Interactions between polymeric nanoparticles and different buffers as investigated by zeta potential measurements and molecular dynamics simulations

Zeta potential is an essential surface parameter in the characterization of nanoparticles, determined at the interface of loosely bound ions (diffuse layer) at the nanoparticle surface and free ions in solution. The ionic concentration and pH of the solution are known to, by definition, influence th...

Full description

Saved in:

Bibliographic Details
Published in:	View (Beijing, China) Vol. 3; no. 4
Main Authors:	Inam, Wali, Bhadane, Rajendra, Akpolat, Rukiye Nur, Taiseer, Rifahul Abrar, Filippov, Sergey K., Salo‐Ahen, Outi M. H., Rosenholm, Jessica M, Zhang, Hongbo
Format:	Journal Article
Language:	English
Published:	Beijing John Wiley & Sons, Inc 01-07-2022 Wiley
Subjects:	Acids buffer interaction Drug delivery systems molecular dynamics simulation Nanomaterials Nanoparticles Physiology polymeric nanoparticles Polymers Simulation zeta potential
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Zeta potential is an essential surface parameter in the characterization of nanoparticles, determined at the interface of loosely bound ions (diffuse layer) at the nanoparticle surface and free ions in solution. The ionic concentration and pH of the solution are known to, by definition, influence the composition of the diffuse layer and zeta potential accordingly. Thus, to fix the solution's pH for valid zeta potential measurements, buffers are frequently used. However, an issue that remains largely neglected is that buffers could also additionally alter the electrokinetic properties of nanoparticles through specific molecular interactions. Therefore, a thorough molecular understanding of buffer–nanoparticle interactions is needed to correctly implement zeta potential results. Thus, in order to study nanoparticle–buffer interactions, we first adopted a simple experimental approach of measuring zeta potential of common polymeric nanoparticle systems at different buffer concentrations, pH, and nanoparticle–buffer fraction ratios. We observed that zwitterionic/cationic buffer molecules impart significant interference to the electrokinetic properties of structurally diverse polymer nanoparticles, by causing zeta potential suppression or even inversion during the experiments. In parallel, advancement in computation resources nowadays allow studying intermolecular interactions of nanoparticles and other complex molecules by molecular dynamics (MD) simulations. Thus, by performing MD simulations for six different polymeric nanomaterials with commonly used buffer molecules, we found that noncovalent interactions play a significant role in altering the observed zeta potential values, which may contribute to erroneous results and false particle characterizations if not taken properly into account in zeta potential measurements. Schematic illustration of the evaluation of polymeric nanoparticle–buffer interactions with the aid of zeta potential. Zeta potential is the measure of the surface electrical potential of a particle due to the charged molecular species surrounding a particle in a solution. In this study, zeta potential was investigated under varying solution conditions and, along with computational modeling approach, to establish that buffer–particle interaction could significantly alter zeta potential values. Thus, buffers must be carefully selected to make reliable zeta potential measurements and particle characterizations.
ISSN:	2688-3988 2688-268X 2688-268X
DOI:	10.1002/VIW.20210009