Search Results - "Akita, Mitsuru"

Three Sets of Translocation Intermediates Are Formed during the Early Stage of Protein Import into Chloroplasts by Inoue, Hitoshi, Akita, Mitsuru

“…During the early stage of protein import into chloroplasts, precursor proteins synthesized in the cytosol irreversibly bind to chloroplasts to form the early…”
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ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro by Nomura, Yuhta, Takabayashi, Taito, Kuroda, Hiroshi, Yukawa, Yasushi, Sattasuk, Kwanchanok, Akita, Mitsuru, Nozawa, Akira, Tozawa, Yuzuru

“…Chloroplasts possess common biosynthetic pathways for generating guanosine 3′,5′-(bis)pyrophosphate (ppGpp) from GDP and ATP by RelA-SpoT homolog enzymes. To…”
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Identification of protein transport complexes in the chloroplastic envelope membranes via chemical cross-linking by Akita, M. (Michigan State University, East Lansing, MI.), Nielsen, E, Keegstra, K

“…Transport of cytoplasmically synthesized proteins into chloroplasts uses an import machinery present in the envelope membranes. To identify the components of…”
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Alternative processing of arabidopsis Hsp70 precursors during protein import into chloroplasts by Ratnayake, R.M.U.(Ehime Univ., Matsuyama (Japan)), Inoue, H, Nonami, H, Akita, M

“…During protein import into chloroplasts, one of the Hsp70 proteins in pea (Hsp70-IAP), previously reported to localize in the intermembrane space of…”
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Development and optimization of an in vitro chloroplastic protein import assay using recombinant proteins by Inoue, Hitoshi, Ratnayake, R.M. Udayangani, Nonami, Hiroshi, Akita, Mitsuru

“…The in vitro protein import experiment is one of the most important techniques for determining protein localization. For chloroplastic proteins, proteins of…”
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Kunitz soybean [Glycine max] trypsin inhibitor is modified at its C-terminus by novel soybean thiol protease (protease T1) by Sugawara, M.(Tokyo Univ. of Agriculture, Abashiri, Hokkaido (Japan). Faculty of Bio-Industry), Ito, D, Yamamoto, K, Akita, M, Oguri, S, Momonoki, Y.S

“…Kunitz soybean trypsin inhibitor (KSTI) is hydrolyzed during seed germination to yield amino acids needed to support initial seedling growth. The type of KSTI…”
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Early Steps in the Biosynthesis of NAD in Arabidopsis Start with Aspartate and Occur in the Plastid by Katoh, Akira, Uenohara, Kazuya, Akita, Mitsuru, Hashimoto, Takashi

“…NAD is a ubiquitous coenzyme involved in oxidation-reduction reactions and is synthesized by way of quinolinate. Animals and some bacteria synthesize…”
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The role of molecular chaperones during protein transport into chloroplasts in higher plants by Akita, Mitsuru

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The transition of early translocation intermediates in chloroplasts is accompanied by the movement of the targeting signal on the precursor protein by Inoue, Hitoshi, Akita, Mitsuru

“…During protein import into chloroplasts, precursor proteins are docked to these organelles under stringent energy conditions to form early translocation…”
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Stimulation of transit-peptide release and ATP hydrolysis by a cochaperone during protein import into chloroplasts by Chou, Ming-Lun, Chu, Chiung-Chih, Chen, Lih-Jen, Akita, Mitsuru, Li, Hsou-min

“…Three components of the chloroplast protein translocon, Tic110, Hsp93 (ClpC), and Tic40, have been shown to be important for protein translocation across the…”
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In Vitro Fluorescent Analysis of Preprotein Import into Chloroplasts by KWANCHANOK, Sattasuk, INOUE, Hitoshi, AKITA, Mitsuru

“…Despite recent progress in fluorescence techniques employed to observe protein localization in living cells, the in vitro chloroplastic protein transport assay…”
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Protein import into chloroplasts: the role of GTP during the early stage of import by Akita, Mitsuru

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SecA protein is directly involved in protein secretion in Escherichia coli by Kawasaki, Hisashi, Matsuyama, Shin-ichi, Sasaki, Shoichi, Akita, Mitsuru, Mizushima, Shoji

“…A high-expression plasmid for the secA gene was constructed. The SecA protein was then overproduced in E. coli and purified. The purified SecA stimulated the…”
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In Vitro Protein Import of a Putative Amino Acid Transporter from Arabidopsis thaliana into Chloroplasts and Its Suborganellar Localization by SATTASUK, Kwanchanok, NOZAWA, Akira, TOZAWA, Yuzuru, KAKINUMA, Yoshimi, AKITA, Mitsuru

“…We identified a gene product of At5g19500 (At5g19500p) from Arabidopsis thaliana that is homologous to EcTyrP, a tyrosine-specific transporter from Escherichia…”
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Molecular chaperones involved in chloroplast protein import by Jackson-Constan, Diane, Akita, Mitsuru, Keegstra, Kenneth

“…Transport of cytoplasmically synthesized precursor proteins into chloroplasts, like the protein transport systems of mitochondria and the endoplasmic…”
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Tic40, a membrane-anchored co-chaperone homolog in the chloroplast protein translocon by Li, Hsou-min, Chou, Ming-Lun, Fitzpatrick, Lynda M, Tu, Shuh-Long, Budziszewski, Gregory, Potter-Lewis, Sharon, Akita, Mitsuru, Levin, Joshua Z, Keegstra, Kenneth

“…The function of Tic40 during chloroplast protein import was investigated. Tic40 is an inner envelope membrane protein with a large hydrophilic domain located…”
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Stable association of chloroplastic precursors with protein translocation complexes that contain proteins from both envelope membranes and a stromal Hsp100 molecular chaperone by Nielsen, E, Akita, M, Davila-Aponte, J, Keegstra, K

“…Cytoplasmically synthesized precursors interact with translocation components in both the outer and inner envelope membranes during transport into…”
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Identification of three shikimate kinase genes in rice: characterization of their differential expression during panicle development and of the enzymatic activities of the encoded proteins by Kasai, K, Kanno, T, Akita, M, Ikejiri-Kanno, Y, Wakasa, K, Tozawa, Y

“…The shikimate pathway is common to the biosynthesis of the three aromatic amino acids and that of various secondary metabolites in land plants. Shikimate…”
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Potent and specific inhibition of mammalian histone deacetylase both in vivo and in vitro by trichostatin A by Yoshida, M, Kijima, M, Akita, M, Beppu, T

“…(R)-Trichostatin A (TSA) is a Streptomyces product which causes the induction of Friend cell differentiation and specific inhibition of the cell cycle of…”
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Early Steps in the Biosynthesis of NAD in Arabidopsis Start with Aspartate and Occur in the Plastid1 by Katoh, Akira, Uenohara, Kazuya, Akita, Mitsuru, Hashimoto, Takashi

“…NAD is a ubiquitous coenzyme involved in oxidation-reduction reactions and is synthesized by way of quinolinate. Animals and some bacteria synthesize…”
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