Search Results - "Akey, David"

Extended surface for membrane association in Zika virus NS1 structure by Brown, W Clay, Akey, David L, Konwerski, Jamie R, Tarrasch, Jeffrey T, Skiniotis, Georgios, Kuhn, Richard J, Smith, Janet L

“…The crystal structure of full-length NS1 protein from Zika virus reveals an extended surface for membrane association and a highly variable polar surface. The…”
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Flavivirus NS1 Structures Reveal Surfaces for Associations with Membranes and the Immune System by Akey, David L., Brown, W. Clay, Dutta, Somnath, Konwerski, Jamie, Jose, Joyce, Jurkiw, Thomas J., DelProposto, James, Ogata, Craig M., Skiniotis, Georgios, Kuhn, Richard J., Smith, Janet L.

“…Flaviviruses, the human pathogens responsible for dengue fever, West Nile fever, tick-borne encephalitis, and yellow fever, are endemic in tropical and…”
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Thiosulfate sulfurtransferase-like domain–containing 1 protein interacts with thioredoxin by Libiad, Marouane, Motl, Nicole, Akey, David L., Sakamoto, Naoya, Fearon, Eric R., Smith, Janet L., Banerjee, Ruma

“…Rhodanese domains are structural modules present in the sulfurtransferase superfamily. These domains can exist as single units, in tandem repeats, or fused to…”
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Structural basis for antibody inhibition of flavivirus NS1-triggered endothelial dysfunction by Biering, Scott B, Akey, David L, Wong, Marcus P, Brown, W Clay, Lo, Nicholas T N, Puerta-Guardo, Henry, Tramontini Gomes de Sousa, Francielle, Wang, Chunling, Konwerski, Jamie R, Espinosa, Diego A, Bockhaus, Nicholas J, Glasner, Dustin R, Li, Jeffrey, Blanc, Sophie F, Juan, Evan Y, Elledge, Stephen J, Mina, Michael J, Beatty, P Robert, Smith, Janet L, Harris, Eva

“…Medically important flaviviruses cause diverse disease pathologies and collectively are responsible for a major global disease burden. A contributing factor to…”
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Structure of a modular polyketide synthase reducing region by McCullough, Tyler M., Dhar, Anya, Akey, David L., Konwerski, Jamie R., Sherman, David H., Smith, Janet L.

“…The chemical scaffolds of numerous therapeutics are polyketide natural products, many formed by bacterial modular polyketide synthases (PKS). The large and…”
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Structure‐guided insights on the role of NS1 in flavivirus infection by Akey, David L, Brown, W.Clay, Jose, Joyce, Kuhn, Richard J, Smith, Janet L

“…We highlight the various domains of the flavivirus virulence factor NS1 and speculate on potential implications of the NS1 3D structure in understanding its…”
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Cryo-EM Analysis Reveals Structural Basis of Helicobacter pylori VacA Toxin Oligomerization by Su, Min, Erwin, Amanda L., Campbell, Anne M., Pyburn, Tasia M., Salay, Lauren E., Hanks, Jessica L., Lacy, D. Borden, Akey, David L., Cover, Timothy L., Ohi, Melanie D.

“…Helicobacter pylori colonizes the human stomach and contributes to the development of gastric cancer and peptic ulcer disease. H. pylori secretes a…”
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Can I solve my structure by SAD phasing? Anomalous signal in SAD phasing by Terwilliger, Thomas C., Bunkóczi, Gábor, Hung, Li-Wei, Zwart, Peter H., Smith, Janet L., Akey, David L., Adams, Paul D.

“…A key challenge in the SAD phasing method is solving a structure when the anomalous signal‐to‐noise ratio is low. A simple theoretical framework for describing…”
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Crystal Structures of Dehydratase Domains from the Curacin Polyketide Biosynthetic Pathway by Akey, David L., Razelun, Jamie R., Tehranisa, Jason, Sherman, David H., Gerwick, William H., Smith, Janet L.

“…Modular polyketide synthases (PKS) make novel natural products through a series of preprogrammed chemical steps catalyzed by an assembly line of multidomain…”
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Can I solve my structure by SAD phasing? Planning an experiment, scaling data and evaluating the useful anomalous correlation and anomalous signal by Terwilliger, Thomas C., Bunkóczi, Gábor, Hung, Li-Wei, Zwart, Peter H., Smith, Janet L., Akey, David L., Adams, Paul D.

“…A key challenge in the SAD phasing method is solving a structure when the anomalous signal‐to‐noise ratio is low. Here, algorithms and tools for evaluating and…”
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Efficient merging of data from multiple samples for determination of anomalous substructure by Akey, David L., Terwilliger, Thomas C., Smith, Janet L.

“…Merging of data from multiple crystals has proven to be useful for determination of the anomalously scattering atomic substructure for crystals with weak…”
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The oligomeric state of CtBP determines its role as a transcriptional co-activator and co-repressor of Wingless targets by Bhambhani, Chandan, Chang, Jinhee L, Akey, David L, Cadigan, Ken M

“…C‐terminal‐binding protein (CtBP) is a well‐characterized transcriptional co‐repressor that requires homo‐dimerization for its activity. CtBP can both repress…”
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Use of massively multiple merged data for low-resolution S-SAD phasing and refinement of flavivirus NS1 by Akey, David L., Brown, W. Clay, Konwerski, Jamie R., Ogata, Craig M., Smith, Janet L.

“…An emergent challenge in macromolecular crystallography is the identification of the substructure from native anomalous scatterers in crystals that diffract to…”
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Context-Dependent Cleavage of the Capsid Protein by the West Nile Virus Protease Modulates the Efficiency of Virus Assembly by VanBlargan, Laura A, Davis, Kaitlin A, Dowd, Kimberly A, Akey, David L, Smith, Janet L, Pierson, Theodore C

“…The molecular mechanisms that define the specificity of flavivirus RNA encapsulation are poorly understood. Virions composed of the structural proteins of one…”
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Biochemical and Structural Characterization of the Tautomycetin Thioesterase: Analysis of a Stereoselective Polyketide Hydrolase by Scaglione, Jamie B, Akey, David L, Sullivan, Rachel, Kittendorf, Jeffrey D, Rath, Christopher M, Kim, Eung-Soo, Smith, Janet L, Sherman, David H

“…A narrow tunnel: Biochemical and structural analysis of the tautomycetin thioesterase (TE) has provided the first high‐resolution structure of a…”
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Biochemical and Structural Characterization of Germicidin Synthase: Analysis of a Type III Polyketide Synthase That Employs Acyl-ACP as a Starter Unit Donor by Chemler, Joseph A, Buchholz, Tonia J, Geders, Todd W, Akey, David L, Rath, Christopher M, Chlipala, George E, Smith, Janet L, Sherman, David H

“…Germicidin synthase (Gcs) from Streptomyces coelicolor is a type III polyketide synthase (PKS) with broad substrate flexibility for acyl groups linked through…”
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Structure and Functional Analysis of RifR, the Type II Thioesterase from the Rifamycin Biosynthetic Pathway by Claxton, Heather B., Akey, David L., Silver, Monica K., Admiraal, Suzanne J., Smith, Janet L.

“…Two thioesterases are commonly found in natural product biosynthetic clusters, a type I thioesterase that is responsible for removing the final product from…”
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Structural basis for macrolactonization by the pikromycin thioesterase by Fecik, Robert A, Sherman, David H, Smith, Janet L, Akey, David L, Kittendorf, Jeffrey D, Giraldes, John W

“…Polyketides are a class of biologically active microbial and plant-derived metabolites that possess a high degree of structural and functional diversity and…”
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Insights from the sea: structural biology of marine polyketide synthases by Akey, David L, Gehret, Jennifer J, Khare, Dheeraj, Smith, Janet L

“…The world's oceans are a rich source of natural products with extremely interesting chemistry. Biosynthetic pathways have been worked out for a few, and the…”
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A 7μm mini-beam improves diffraction data from small or imperfect crystals of macromolecules by Sanishvili, Ruslan, Nagarajan, Venugopalan, Yoder, Derek, Becker, Michael, Xu, Shenglan, Corcoran, Stephen, Akey, David L, Smith, Janet L, Fischetti, Robert F

“…A simple apparatus for achieving beam sizes in the range 5-10 μm on a synchrotron beamline was implemented in combination with a small 125 x 25 μm focus. The…”
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