Effects of glycerol additons on post-thaw fertility of frozen rainbow trout sperm, with an emphasis on interaction between extender and cryoprotectant
The study was conducted to evaluate the effects of different extenders, cryoprotectants and glycerol additions on the postthaw fertility and interactions between extenders and cryoprotectants during cryopreservation. Semen was collected by abdominal stripping from 30 adult male rainbow trout (Oncorh...
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| Published in: | Journal of applied ichthyology Vol. 23; no. 1; pp. 60 - 63 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-01-2007
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | The study was conducted to evaluate the effects of different extenders, cryoprotectants and glycerol additions on the postthaw fertility and interactions between extenders and cryoprotectants during cryopreservation. Semen was collected by abdominal stripping from 30 adult male rainbow trout (Oncorhynchus mykiss Walbaum, 1792) and diluted with three different extenders (Erdahl-Graham, Lahnsteiner, glucosebased) containing 15% DMA, 15% DMSO, 15% DMA + 1% glycerol and 15% DMSO + 1% glycerol at a ratio of 1 : 2. Diluted samples were frozen as 0.1 ml pellets directly an dry ice (solid CO sub(2), -79 degree C). Eggs were pooled from 10 females. Fertilization was applied in plastic dishes and 600 eggs were used in each fertilization trial. Pellets were thawed in their own extenders (30 degree C) at a ratio of 1 : 10. 0.3 degree /o NaCI was used for activating motility. Sperm-egg ratio was approximately 3 x 10 super(6) sp per egg. Experimental success was determined as the percentage of eyed-eggs 25 days alter fertilization. The highest eyed-egg rate (49.3%) was obtained from semen frozen with glucose-based extender containin,- 15% DMSO - 1 % glycerol. Our resutts indicate that the glucose-based extender containing DMSO is a useful combination, but that the addition of glycerol does not have a positive effect on postthaw fertility, and that interaction of the extender-cryoprotectant is also important in the cryopreservation of rainbow trout semen. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 content type line 23 ObjectType-Feature-2 |
| ISSN: | 0175-8659 1439-0426 |
| DOI: | 10.1111/j.1439-0426,2006.00792.x |