Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution

In this paper, the development of compact transmission soft x-ray microscopy (XM) with sub-50 nm spatial resolution for biomedical applications is described. The compact transmission soft x-ray microscope operates at lambda = 2.88 nm (430 eV) and is based on a tabletop regenerative x-ray source in c...

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Bibliographic Details
Published in:	Physics in medicine & biology Vol. 51; no. 6; p. N99
Main Authors:	Kim, Kyong Woo, Kwon, Youngman, Nam, Ki-Yong, Lim, Jong-Hyeok, Kim, Kyu-Gyum, Chon, Kwon Su, Kim, Byoung Hoon, Kim, Dong Eon, Kim, JinGon, Ahn, Byoung Nam, Shin, Hyun Joon, Rah, Seungyu, Kim, Ki-Ho, Chae, Jin Seok, Gweon, Dae Gab, Kang, Dong Woo, Kang, Sung Hoon, Min, Jin Young, Choi, Kyu-Sil, Yoon, Seong Eon, Kim, Eun-A, Namba, Yoshiharu, Yoon, Kwon-Ha
Format:	Journal Article
Language:	English
Published:	England 21-03-2006
Subjects:	Diatoms - ultrastructure Electron Probe Microanalysis - methods Image Processing, Computer-Assisted Lasers Microscopy Microscopy, Electron, Transmission - methods Optical Devices Photons Software X-Rays
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Summary:	In this paper, the development of compact transmission soft x-ray microscopy (XM) with sub-50 nm spatial resolution for biomedical applications is described. The compact transmission soft x-ray microscope operates at lambda = 2.88 nm (430 eV) and is based on a tabletop regenerative x-ray source in combination with a tandem ellipsoidal condenser mirror for sample illumination, an objective micro zone plate and a thinned back-illuminated charge coupled device to record an x-ray image. The new, compact x-ray microscope system requires the fabrication of proper x-ray optical devices in order to obtain high-quality images. For an application-oriented microscope, the alignment procedure is fully automated via computer control through a graphic user interface. In imaging studies using our compact XM system, a gold mesh image was obtained with 45 nm resolution at x580 magnification and 1 min exposure. Images of a biological sample (Coscinodiscus oculoides) were recorded.
ISSN:	0031-9155
DOI:	10.1088/0031-9155/51/6/N01